PCR-based detection of Mycobacterium avium subsp. paratuberculosis infection in cattle in South Korea using fecal samples
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- 資料種別
- 記事
- 著者・編者
- Hong-Tae PARKMin-Kyoung SHINHyun-Eui PARKYong-Il CHOHan Sang YOO
- タイトル（掲載誌）
- The journal of veterinary medical science = 日本獣醫學会会誌
- 巻号年月日等（掲載誌）
- 78(9):2016.9
- 掲載巻
- 78
- 掲載号
- 9
- 掲載ページ
- 1537-1540
- 掲載年月日（W3CDTF）
- 2016-09
- ISSN（掲載誌）
- 0916-7250
- ISSN-L（掲載誌）
- 0916-7250
- 出版事項（掲載誌）
- 東京 : 日本獣医学会
- 出版地（国名コード）
- JP
- 本文の言語コード
- eng
- NDLC
- 対象利用者
- 一般
- 所蔵機関
- 国立国会図書館
- 請求記号
- Z18-350
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- 書誌ID（NDLBibID）
- 027686250
- 整理区分コード
- 632
- 要約等
- <p><i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) is the causative agent of bovine paratuberculosis (PTB). The first step in the control of PTB is the identification and isolation of sub-clinical fecal shedders from the herd. In the current study, real-time and nested PCR targeting MAP-specific genetic elements (<i>IS900</i> and <i>ISMAP02</i>) DNA isolated from fecal samples were used to detect MAP infection in cattle. Of the 1,562 fecal samples obtained from 37 herds, regardless of diarrhea, 35 samples tested positive in both <i>IS900</i>-targeted real-time and <i>ISMAP02</i>-targeted nested PCR. At the herd level, 12 of the 37 herds were found to be positive for MAP. Detection rates of the PCR tests were similar to those reported for ELISA-based methods. These results suggest that PCR can be used to detect sub-clinical fecal shedders of MAP.</p>
- DOI
- 10.1292/jvms.15-0271
- オンライン閲覧公開範囲
- インターネット公開
- 連携機関・データベース
- 科学技術振興機構 : J-STAGE
- 要約等
- <p><i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (MAP) is the causative agent of bovine paratuberculosis (PTB). The first step in the control of PTB is the identification and isolation of sub-clinical fecal shedders from the herd. In the current study, real-time and nested PCR targeting MAP-specific genetic elements (<i>IS900</i> and <i>ISMAP02</i>) DNA isolated from fecal samples were used to detect MAP infection in cattle. Of the 1,562 fecal samples obtained from 37 herds, regardless of diarrhea, 35 samples tested positive in both <i>IS900</i>-targeted real-time and <i>ISMAP02</i>-targeted nested PCR. At the herd level, 12 of the 37 herds were found to be positive for MAP. Detection rates of the PCR tests were similar to those reported for ELISA-based methods. These results suggest that PCR can be used to detect sub-clinical fecal shedders of MAP.</p>
- DOI
- 10.1292/jvms.15-0271
- 関連情報（URI）
- 参照
- Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>in Bovine Fecal SamplesAnalysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in KoreaMycobacterium avium subsp. paratuberculosis viability determination using F57 quantitative PCR in combination with propidium monoazide treatmentDevelopment and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samplesIsolation and detection of Mycobacterium avium subsp. paratuberculosis (MAP) from cattle in Ireland using both traditional culture and molecular based methodsEstimation of Paratuberculosis Prevalence in Dairy Cattle in a Province of Korea using an Enzyme-linked Immunosorbent Assay: Application of Bayesian ApproachDetection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and cultureCharacterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing The GenBank accession numbers for the sequences reported in this paper are AJ011838, AJ250015–AJ250023 and AJ251434–AJ251437.Identification of restriction fragment length polymorphisms in DNA from Mycobacterium paratuberculosisInhibition of PCR amplification by phytic acid, and treatment of bovine fecal specimens with phytase to reduce inhibitionHerd-level economic losses associated with Johne's disease on US dairy operationsEffective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine fecesMycobacterium avium subsp. paratuberculosis in cow bulk tank milk in Cyprus detected by culture and quantitative IS900 and F57 real-time PCRDetection of Mycobacterium avium subspecies paratuberculosis in Swiss dairy cattle by real-time PCR and culture: a comparison of the two assaysEvaluation of multiple genomic targets for identification and confirmation of Mycobacterium avium subsp. paratuberculosis isolates using real-time PCRReal-time PCR, compared to liquid and solid culture media and ELISA, for the detection of Mycobacterium avium ssp. paratuberculosisDevelopment and Application of Quantitative Polymerase Chain Reaction Assay Based on the ABI 7700 System (TaqMan) for Detection and Quantification of <i>Mycobacterium Avium</i> Subsp. <i>Paratuberculosis</i>Development and evaluation of a Mycobacterium avium subspecies paratuberculosis (MAP) specific multiplex PCR assaySeroprevalence of <i>Mycobacterium avium</i> subspecies <i>paratuberculosis</i> in cattle in KoreaDevelopment of a Nested PCR Method Targeting a Unique Multicopy Element, ISMap<i>02</i>, for Detection of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>in Fecal SamplesSeroprevalence of Mycobacterium avium subspecies paratuberculosis in Korean Black Goats (Capra hircus aegagrus)
- 連携機関・データベース
- 国立情報学研究所 : CiNii Research
- 提供元機関・データベース
- Japan Link Center雑誌記事索引データベースCrossrefPubMedCiNii ArticlesCiNii Articles
- 書誌ID（NDLBibID）
- 027686250
- NII論文ID
- 13000559809540020976677