下痢原性大腸菌食中毒事例におけるO血清群およびH型の遺伝子型判定PCR検査法の活用

• 資料種別: 記事
• タイトル: 下痢原性大腸菌食中毒事例におけるO血清群およびH型の遺伝子型判定PCR検査法の活用
• 著者・編者: 小田切 正昭; 加藤 直樹; 曽根 美紀; 土屋 彰彦; 近藤 貴英; 井口 純
• 著者標目: 小田切 正昭; 加藤 直樹; 曽根 美紀; 土屋 彰彦; 近藤 貴英; 井口 純
• 並列タイトル等: Escherichia coli O-genotyping PCR and H-genotyping PCR Methods on Food Poisoning of Diarrheagenic E. coli
• タイトル（掲載誌）: 日本食品微生物学会雑誌 = Japanese journal of food microbiology / 日本食品微生物学会編集委員会 [編]
• 巻号年月日等（掲載誌）: 41(3):2024
• 掲載巻: 41
• 掲載号: 3
• 掲載ページ: 119-123
• 掲載年月日（W3CDTF）: 2024
• ISSN（掲載誌）: 1340-8267
• ISSN-L（掲載誌）: 1340-8267
• 出版事項（掲載誌）: 東京 : 日本食品微生物学会
• 出版地（国名コード）: JP
• 本文の言語コード: jpn
• 件名標目: Diarrheagenic Escherichia coli; Og104:Hg4; E. coli O-genotyping PCR and E. coli H-genotyping PCR
• NDLC: ZS17
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z19-1644
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 033766791; http://id.ndl.go.jp/bib/033766791
• 整理区分コード: 632

• 要約等: <p>Diarrheagenic <i>Escherichia coli</i> strains are mainly classified into the following five pathotypes; enteropathogenic <i>E. coli</i>, enterotoxigenic <i>E. coli</i>, enteroinvasive <i>E. coli</i>, enterohemorrhagic <i>E. coli</i> and enteroaggregative <i>E. coli</i>. <i>E. coli</i> strains of various pathotypes can be further classified by their serotypes combined by O-serogroup and H-type. Generally in Japan, the commercial O-antisera and H-antisera are used to serotype strains. These serotypes provide useful information in investigations of outbreaks and epidemiological studies. In May 2021, a large-scale food poisoning outbreak thought to be caused by <i>E. coli</i> occurred in Saitama City. Serotyping of 21 <i>E. coli</i> strains carrying virulence genes isolated from patients and an asymptomatic cooking worker was carried out using a commercially available kit, but most of the strains (57%) could not be serotyped. Therefore, in this study, we tried the multiplex PCR methods including <i>E. coli</i> O-genotyping PCR and <i>E. coli</i> H-genotyping PCR. As a result, only 5% of strains could not be determined their Og-types, and the main genotype in this case was found to be Og104:Hg4 (12/21 strains) carrying <i>astA</i>+<i>aggR</i> or <i>estA2</i>+<i>elt</i>+<i>astA</i>+<i>aggR</i>. In conclusion, we believe that Og-typing PCR and Hg-typing PCR are effective methods in investigating cases caused by pathogenic <i>E. coli</i> belonging to rare pathotypes.</p>
• DOI: 10.5803/jsfm.41.119
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: <p>Diarrheagenic <i>Escherichia coli</i> strains are mainly classified into the following five pathotypes; enteropathogenic <i>E. coli</i>, enterotoxigenic <i>E. coli</i>, enteroinvasive <i>E. coli</i>, enterohemorrhagic <i>E. coli</i> and enteroaggregative <i>E. coli</i>. <i>E. coli</i> strains of various pathotypes can be further classified by their serotypes combined by O-serogroup and H-type. Generally in Japan, the commercial O-antisera and H-antisera are used to serotype strains. These serotypes provide useful information in investigations of outbreaks and epidemiological studies. In May 2021, a large-scale food poisoning outbreak thought to be caused by <i>E. coli</i> occurred in Saitama City. Serotyping of 21 <i>E. coli</i> strains carrying virulence genes isolated from patients and an asymptomatic cooking worker was carried out using a commercially available kit, but most of the strains (57%) could not be serotyped. Therefore, in this study, we tried the multiplex PCR methods including <i>E. coli</i> O-genotyping PCR and <i>E. coli</i> H-genotyping PCR. As a result, only 5% of strains could not be determined their Og-types, and the main genotype in this case was found to be Og104:Hg4 (12/21 strains) carrying <i>astA</i>+<i>aggR</i> or <i>estA2</i>+<i>elt</i>+<i>astA</i>+<i>aggR</i>. In conclusion, we believe that Og-typing PCR and Hg-typing PCR are effective methods in investigating cases caused by pathogenic <i>E. coli</i> belonging to rare pathotypes.</p>
• DOI: 10.5803/jsfm.41.119; https://doi.org/10.5803/jsfm.41.119
• 関連情報（URI）: http://id.ndl.go.jp/bib/033766791; https://ndlsearch.ndl.go.jp/books/R000000004-I033766791; https://www.jstage.jst.go.jp/article/jsfm/41/3/41_119/_pdf; https://search.jamas.or.jp/link/ui/2025043596
• 参照: Atypical diarrhoeagenic <i>Escherichia coli</i> in milk related to a large foodborne outbreak; https://cir.nii.ac.jp/crid/1360583647575621120; Additional Og-Typing PCR Techniques Targeting Escherichia coli-Novel and <i>Shigella</i> -Unique O-Antigen Biosynthesis Gene Clusters; https://cir.nii.ac.jp/crid/1360853567675877248; Diarrheagenic<i>Escherichia coli</i>; https://cir.nii.ac.jp/crid/1360855569142487296; Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping; https://cir.nii.ac.jp/crid/1362825894186831488; Prevalence and Characteristics of Shiga Toxin-Producing <i>Escherichia coli</i> from Healthy Cattle in Japan; https://cir.nii.ac.jp/crid/1364233270473218304; 散発下痢症患者由来大腸菌の腸管病原性大腸菌 (EPEC) <I>eaeA</I>遺伝子および腸管凝集性大腸菌 (EAggEC) <I>aggR</I>遺伝子保有状況とその病原性の評価; https://cir.nii.ac.jp/crid/1390001205050267264; 大腸菌のO血清群とO抗原とO抗原合成遺伝子; https://cir.nii.ac.jp/crid/1390282679402775680; 腸管集合性大腸菌耐熱性エンテロトキシン-1 (EAST-1) 遺伝子を保有する, 食中毒様事例由来病原血清型大腸菌; https://cir.nii.ac.jp/crid/1390282680023583616
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org
• 書誌ID（NDLBibID）: 033766791; http://id.ndl.go.jp/bib/033766791