STEROIDAL SAPONIN IN ETHANOL EXTRACT TUBER OF PURPLE YAM (Dioscorea alata L.) AS ALLERGENIC AGENT ON SENSITIVITY PHASE BALB/c MICE MODEL ALLERGY

• 資料種別: 文書・図像類
• タイトル: STEROIDAL SAPONIN IN ETHANOL EXTRACT TUBER OF PURPLE YAM (Dioscorea alata L.) AS ALLERGENIC AGENT ON SENSITIVITY PHASE BALB/c MICE MODEL ALLERGY
• 著者・編者: MAKIYAH, Sri Nabawiyati Nurul; TASMINATUN, Sri; RIFA’I, Muhaimin; WIDODO; DJATI, Muhammad Sasmito
• 著者標目: MAKIYAH, Sri Nabawiyati Nurul; TASMINATUN, Sri; RIFA’I, Muhaimin; WIDODO; DJATI, Muhammad Sasmito
• 出版事項: Chiba University. Center for Environmental Remote Sensing
• 出版年月日等: 2016-11-21
• 出版年（W3CDTF）: 2016-11-21
• タイトル（掲載誌）: Proceedings of the CEReS international symposium = CEReS国際シンポジウム資料集
• 巻号年月日等（掲載誌）: 24
• 掲載巻: 24
• 掲載ページ: 175-184
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: 出版タイプ： VoR; type:text; [ABSTRACT]Purple yam (Dioscorea alata L.) is a source of biological tubers not been used optimally. Diosgenin is steroids saponin compounds that most important because it has multiple biological functions, such as allergenic activity. The objective of this research was to analyze the potential allergenic ethanol extract tuber of D. alata L. (EEDA) on BALB/c mice on sensitivity phase with measure the profile of transcription factor FoxP3 Treg cells and cytokine profile of Treg and IgE and IgG1 B cells. An experimental study using BALB/c mice divided into 7 groups: control group (I), the treatment group (II-V) ethanol extract of tuber Dioscorea alata L. dose of 0.00; 0.17; 2.01; 10.04 g/kg bw, the treatment group antihistamines drug and Diosgenin (VI-VII). For 17 consecutive days the group II-VII were treated in accordance with the group and with Ovalbumin induced allergy models. Mice were sacrificed on day 18. Spleen is removed, lymphocyte isolated and analyze the transcription factor FoxP3 Treg cells, cytokine profile of T reg cells and IgE and IgG1 B cells on spleen using Flowcytometry FACS Calibur. The results showed EEDA able to inhibit the production of B220IgE and B220IgG1, trigger Treg cells (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and profile of the cytokines produced by T reg cells. The conclusion is ethanol extract tubers of Dioscorea alata L (EEDA) does not trigger Treg (CD4CD25) and the transcription factor FoxP3 (CD4CD25FoxP3) and cytokine profiles produced by Treg cells namely CD4IL-10, CD8IL-10, CD4TGF-β and CD8TGF-β in the sensitivity phase.
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 千葉大学 : 千葉大学学術成果リポジトリ