THE SIGNIFICANCE OF GLUTAMIC DEHYDROGENASE IN GLUTAMIC ACID FERMENTATION
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- 資料種別
- 記事
- 著者標目
- 出版年月日等
- 1962
- 出版年(W3CDTF)
- 1962
- タイトル(掲載誌)
- The Journal of General and Applied Microbiology
- 巻号年月日等(掲載誌)
- 8 4
- 掲載巻
- 8
- 掲載号
- 4
- 掲載ページ
- 253-260
- 掲載年月日(W3CDTF)
- 1962
- ISSN(掲載誌)
- 00221260
- ISSN-L(掲載誌)
- 00221260
- 出版事項(掲載誌)
- Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
- 本文の言語コード
- en
- 対象利用者
- 一般
- DOI
- 10.2323/jgam.8.253
- 連携機関・データベース
- 国立情報学研究所 : CiNii Research
- 提供元機関・データベース
- Japan Link CenterCrossrefCiNii Articles
- NII論文ID
- 130003458820
- 要約等
- 1. The activity to synthesize aspartic acid from fumaric acid and ammonia and the activity to synthesize glutamic acid from citric acid and ammonia were examined with the cell-free extracts obtained from several strains of <i>M. glutamicus</i> and their mutants grown on glucose bouillon media. All of them were found to possess a strong activity of the latter but practically no activity of the former.<br>2. The activities of aspartase, glutamic dehydrogenase, alanine dehydrogenase, leucine dehyrogenase, glutamic-aspartic transaminase, and glutamic-alanine transaminase were examined with the cell-free extracts obtained from the cells of <i>M. glutamicus</i> (parent strain) grown on different levels of biotin. Only glutamic dehydrogenase and transaminases were detected in ample amounts. No significant difference was found in the activities of enzymes involved in the ammonia uptake in the extracts of the cells grown on the different levels of biotin. <br>3. From the results 1 and 2, it is concluded that in <i>M. glutamicus</i> the principal mechanism of ammonia uptake is effected by TPN-linked glutamic dehydrogenase irrespective of the biotin level in the growth medium, and consequently, the biosynthesis of the amino acids belonging to glutamic acid family and aspartic acid family proceeds directly or indirectly via the synthesis of glutamic acid.
- DOI
- 10.2323/jgam.8.253
- オンライン閲覧公開範囲
- インターネット公開
- 連携機関・データベース
- 科学技術振興機構 : J-STAGE