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THE SIGNIFICANCE OF GLUTAMIC DEHYDROGENASE IN GLUTAMIC ACID FERMENTATION

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THE SIGNIFICANCE OF GLUTAMIC DEHYDROGENASE IN GLUTAMIC ACID FERMENTATION

資料種別
記事
著者
KIMURA KAZUO
出版者
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
出版年
1962
資料形態
デジタル
掲載誌名
The Journal of General and Applied Microbiology 8 4
掲載ページ
p.253-260
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資料詳細

要約等:

1. The activity to synthesize aspartic acid from fumaric acid and ammonia and the activity to synthesize glutamic acid from citric acid and ammonia we...

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デジタル

資料種別
記事
著者標目
出版年月日等
1962
出版年(W3CDTF)
1962
タイトル(掲載誌)
The Journal of General and Applied Microbiology
巻号年月日等(掲載誌)
8 4
掲載巻
8
掲載号
4
掲載ページ
253-260
掲載年月日(W3CDTF)
1962
ISSN(掲載誌)
00221260
ISSN-L(掲載誌)
00221260
出版事項(掲載誌)
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
本文の言語コード
en
対象利用者
一般
連携機関・データベース
国立情報学研究所 : CiNii Research
提供元機関・データベース
Japan Link Center
Crossref
CiNii Articles
NII論文ID
130003458820

デジタル

要約等
1. The activity to synthesize aspartic acid from fumaric acid and ammonia and the activity to synthesize glutamic acid from citric acid and ammonia were examined with the cell-free extracts obtained from several strains of <i>M. glutamicus</i> and their mutants grown on glucose bouillon media. All of them were found to possess a strong activity of the latter but practically no activity of the former.<br>2. The activities of aspartase, glutamic dehydrogenase, alanine dehydrogenase, leucine dehyrogenase, glutamic-aspartic transaminase, and glutamic-alanine transaminase were examined with the cell-free extracts obtained from the cells of <i>M. glutamicus</i> (parent strain) grown on different levels of biotin. Only glutamic dehydrogenase and transaminases were detected in ample amounts. No significant difference was found in the activities of enzymes involved in the ammonia uptake in the extracts of the cells grown on the different levels of biotin. <br>3. From the results 1 and 2, it is concluded that in <i>M. glutamicus</i> the principal mechanism of ammonia uptake is effected by TPN-linked glutamic dehydrogenase irrespective of the biotin level in the growth medium, and consequently, the biosynthesis of the amino acids belonging to glutamic acid family and aspartic acid family proceeds directly or indirectly via the synthesis of glutamic acid.
DOI
10.2323/jgam.8.253
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