マカクの付着系細胞におけるin vitro加齢変化

• Material Type: 記事
• Title: マカクの付着系細胞におけるin vitro加齢変化
• Author/Editor: 清水 裕子
• Author Heading: 清水 裕子
• Periodical title: 霊長類研究 = Primate research
• No. or year of volume/issue: 16(2) 2000.10
• Volume: 16
• Issue: 2
• Pages: 77～85
• Publication date of volume/issue (W3CDTF): 2000-10
• ISSN (Periodical Title): 0912-4047
• ISSN-L (Periodical Title): 0912-4047
• Publication (Periodical Title): 東京 : 日本霊長類学会
• Place of Publication (Country Code): JP
• Text Language Code: jpn
• NDLC: ZR4
• Target Audience: 一般
• Holding library: 国立国会図書館
• Call No.: Z18-1898
• Data Provider (Database): 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• Bibliographic ID (NDL): 5537220; http://id.ndl.go.jp/bib/5537220
• Bibliographic Record Category (NDL): 632

• Summary, etc.: Human fibroblasts have a limited replicative lifespan and never undergo infinite cell division <i>in vitro</i>. In contrast, rodent fibroblasts spontaneously and highly frequently immortalize <i>in vitro</i>. Therefore, rodent is inappropriate as a model animal to study human aging <i>in vitro</i>. To test effects of macaque monkey as the model system, macaque adherent cells were cultured and passaged <i>in vitro</i> and analyzed cytologically.<br>Long-tailed macaque (<i>Macaca fascicularis</i>), Japanese macaque (<i>Macaca fuscata</i>), and bonnet monkey (<i>Macaca radiata</i>) were subjected to the study. Adherent cells were isolated from their skin, kidney, and lung. A total of 19 cell cultures were examined until terminating cell division.<br>Most of the cultures (17/19) exhibited senescence by 7-25 Population Doubling Levels (PDLs), showing enlargement of cell size, decrease of saturation density and extension of doubling interval, and then terminated cell division [Mortality stage 1 (M1)]. The remaining two cell cultures showed distinct pattern. They first exhibited senescent morphology at around 20PDLs, but continued cell division through M1 up to 106 PDLs and then went into crisis stage [Mortality stage 2 (M2)]. In all cell cultures tested, telomerase activity was not detected. Consistently, telomeres appeared to be shortened by every PDLs.<br>Macaque cells showed an intermediate pattern of <i>in vitro</i> aging between human and rodent cells, whereas, they showed no telomerase activity similarly to human cells. Therefore, the macaques must serve as an excellent animal model to study human cellular aging and provide us with a key to study the mechanism of the transition to critical stages in cellular aging.
• DOI: 10.2354/psj.16.77
• Access Restrictions: インターネット公開
• Data Provider (Database): 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• Summary, etc.: Human fibroblasts have a limited replicative lifespan and never undergo infinite cell division <i>in vitro</i>. In contrast, rodent fibroblasts spontaneously and highly frequently immortalize <i>in vitro</i>. Therefore, rodent is inappropriate as a model animal to study human aging <i>in vitro</i>. To test effects of macaque monkey as the model system, macaque adherent cells were cultured and passaged <i>in vitro</i> and analyzed cytologically.<br>Long-tailed macaque (<i>Macaca fascicularis</i>), Japanese macaque (<i>Macaca fuscata</i>), and bonnet monkey (<i>Macaca radiata</i>) were subjected to the study. Adherent cells were isolated from their skin, kidney, and lung. A total of 19 cell cultures were examined until terminating cell division.<br>Most of the cultures (17/19) exhibited senescence by 7-25 Population Doubling Levels (PDLs), showing enlargement of cell size, decrease of saturation density and extension of doubling interval, and then terminated cell division [Mortality stage 1 (M1)]. The remaining two cell cultures showed distinct pattern. They first exhibited senescent morphology at around 20PDLs, but continued cell division through M1 up to 106 PDLs and then went into crisis stage [Mortality stage 2 (M2)]. In all cell cultures tested, telomerase activity was not detected. Consistently, telomeres appeared to be shortened by every PDLs.<br>Macaque cells showed an intermediate pattern of <i>in vitro</i> aging between human and rodent cells, whereas, they showed no telomerase activity similarly to human cells. Therefore, the macaques must serve as an excellent animal model to study human cellular aging and provide us with a key to study the mechanism of the transition to critical stages in cellular aging.
• DOI: 10.2354/psj.16.77; https://doi.org/10.2354/psj.16.77
• Access Restrictions: インターネット公開
• Related Material (URI): http://id.ndl.go.jp/bib/5537220; https://ndlsearch.ndl.go.jp/books/R000000004-I5537220
• Data Provider (Database): 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• Original Data Provider (Database): Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• Bibliographic ID (NDL): 5537220; http://id.ndl.go.jp/bib/5537220
• NAID: 10010164979