Expression dynamics of podoplanin in the mineralization of cultured osteoblasts with mechanostress

• Material Type: 博士論文
• Title: Expression dynamics of podoplanin in the mineralization of cultured osteoblasts with mechanostress
• Author/Editor: 竹縄, 智紘
• Author Heading: 竹縄, 智紘
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2018-03-22
• Publication Date (W3CDTF): 2018-03-22
• Alternative Title: 機械的ストレスを負荷した培養骨芽細胞の石灰化におけるポドプラニンの発現動態
• Degree grantor/type: 北海道大学
• Date Granted: 2018-03-22
• Date Granted (W3CDTF): 2018-03-22
• Dissertation Number: 甲第13041号
• Degree Type: 博士(歯学)
• Text Language Code: eng
• Subject Heading: podoplanin; mechanostress; mineralization; anti-podoplanin
• NDC: 497
• Target Audience: 一般
• Note (General): Here, we report the expression dynamics of podoplanin in cultured bone marrow-derived osteoblasts with mechanical stress and the effect of anti-podolanin on the mineralization. The expression of podoplanin in cultured osteoblasts subjected to vertical elongation straining was significantly higher than that in cells without straining, and the expression increased with duration of elongation time as well as the expression for both osteopontin and osteocalcin tested as positive controls. The podoplanin mRNA amount in cultured osteoblasts subjected to straining in non-mineralization medium was significantly larger than in unstrained cells in mineralization medium. In the western blot analysis for the immunoprecipitated protein and in ELISA for the cell lysate, cultured osteoblasts increased podoplanin production with longer durations of elongation straining and reached a plateau within 3-5 days. There are significantly less of mineralization products in osteoblasts cultured with antibodies for podoplanin, as well as in cells with both antibodies for osteopontin, and osteocalcin as positive controls than in cells with medium only and with phenotype antibody controls. These findings may suggest that mechanostress induces the production of podoplanin, and that podoplanin may contribute to the mineralization in osteoblasts in bone under the circumstances with mechanostress.; (主査) 特任教授 飯田 順一郎, 教授 網塚 憲生, 特任教授 鈴木 邦明; 歯学研究科（口腔医学専攻）
• DOI: 10.14943/doctoral.k13041; https://doi.org/10.14943/doctoral.k13041
• Persistent ID (NDL): info:ndljp/pid/12886225; https://dl.ndl.go.jp/pid/12886225
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2023-06-02T22:06:10+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k13041; http://hdl.handle.net/2115/89194
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Here, we report the expression dynamics of podoplanin in cultured bone marrow-derived osteoblasts with mechanical stress and the effect of anti-podola...