Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat / Katsuro Furukawa [著]

Tue, 21 Oct 2014 20:02:10 +0900

Katsuro Furukawa [著]. Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat. [Katsuro Furukawa], [2014]

Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat / 古川, 克郎

Thu, 24 Aug 2023 18:50:34 +0900

Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat 46 6 / 古川, 克郎

Thu, 30 Oct 2025 00:21:45 +0900

古川, 克郎. Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat 46 6. 日本組織細胞化学会, 2014-02-05; 46 6
Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF instillation with low (0.4 mg/kg) and high (4 mg/kg) doses at various time-points. The proliferation of alveolar cells was assessed by the immunostaining for proliferating cell nuclear antigen (PCNA) in the residual lung. We also investigated other immunohistochemical parameters such as KGF, KGF receptor and surfactant protein A as well as terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. Consequently, intratracheal single injection of rhKGF in high dose group significantly increased PCNA labeling index (LI) of alveolar cells in the remaining lung. Surprisingly, there was no difference in PCNA LI between low and high doses of rhKGF with daily injection, and PCNA LI reached a plateau level with 2 days-consecutive administration (about 60%). Our results indicate that even at low dose, daily intratracheal injection is effective to maintain high proliferative states during the early phase of compensatory lung growth.

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Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat / Katsuro Furukawa [著]

Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat / 古川, 克郎

Intratracheal administration of recombinant human keratinocyte growth factor promotes alveolar epithelial cell proliferation during compensatory lung growth in rat 46 6 / 古川, 克郎