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Paper図書
National Diet Library
- Summary, etc....t advancements in mucin research. The chapters in this book are organized into 8 parts and ......ics such as mucin extraction, is......d experimental methods. The chapters ......iscuss the origins of mucins in jellyfish, fec......analysis of mucin gene expressio...... of genes; imaging of mucin networks by AF......d experimental methods using supported molecular matrix electro......resis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Mucins: Methods and Protocols is a valuable r...
- ContentsPreparation of Jellyfish Mucins Extraction of Mucin from Rodent Feces and Determination of O-Linked Oligosaccharide Chain Equivalent Der...... from Fecal Mucin Preparation of Soluble Mucin Solutions from......brane Bound Mucins from Human Br......man Gastric Mucins from Gastric ......traction of Mucins from the Mammalian Intestinal Tract Supported Molecular Matrix Electro......stochemical Staining of Mucins Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluori......titation of Mucin by Densitometr......Alcian Blue-Stained Membrane Qua......e Isotope Labeling Mass Spectrom......lycans from Mucins using Hydrazine Treatment El...
- Subject HeadingMucins--Laboratory manuals Mucines--Manuels de laboratoire
Paper図書
National Diet Library
- Summary, etc....zed analytical methods on a wide rang......ipids. From sphingoglycolipids t......mulant present in lipopolysaccha......he book delves into how glycolip......een organisms. In addition, glyc......important role in infections in animals and ot......nisms by targeting host cell mem......nsidered as living organisms. Wr......hly successful Methods in Molecular Biology series, chapters include introduction to t......on troubleshooting and avoiding known pitfall......, Glycolipids: Methods and Protocols ......lipid research in an effort to help researchers...
- Contents... Synthesis and Molecular Interaction Analy......erial Lipid As Innovative Vaccine Strategy: Self-Adjuvanting Conjugate Vaccines Chemical Syn......truction of Sphingolipid Remodel...... by Genome Editing Mass Spectrom......eutral Glycosphingolipids Struct......he Glycolipids in Lipid Rafts Ex......Microbiota Strains NMR Analysis ......of Glycolipids in the Plasma Mem......pecific Probes in Combination with Sodi......e Replica Labeling (SDS-FRL) Bio......nalyses for Sphingolipids and It......ated Molecules in Phagosomes Single-Molecule Imaging of Ganglioside Probes in Living Cell Plasma M......ion of Enzymes Involved in Glycolipid Metabolis...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2613 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....t experiments, methods, and applicati......anopore sequencing. Chapters gui......ers through bioinformatic proced...... genome sequencing, analysis of ......lysis. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comp...
- Contents...anopore sequencing Hybrid genome......and long reads in Galaxy Microbi...... genome sequencing and assembly using nanopore sequ......modification using nanopore sequ......cers Ultra-low input genome libr......anopore sequencing with droplet-...... method of eliminating the Wolbachia......t genomes from insect samples pr......ng-read sequencing A nanopore sequencing course for gr......uide to sequencing for long repe......t expansions using long DNA reads Finding rearrangements in nanopore DNA r......-genome sequencing using a nanopore se......tural variants in cancer genomes......ene analysis using long-read nanopore sequencin...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2632 Springer protocols (Series)
Paper図書
National Diet Library
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2803. 1064-3745 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....apeutics, covering therapeutics using antisense oli......nt, genome editing, small molecu......ten by leaders in the field, the...... are currently in use and are starting an exciting therapeutic revolution in muscular dystr......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall...... Therapeutics: Methods and Protocols ......al resource to inspire readers and provide tips...
- Contents...sen: From Preclinical Studies to......val Rapid Freezing of Skeletal a......diac Muscles Using Isopentane Co......etic, and Protein Expression Stu......scle Pathology in the D2/mdx Mou......ation of Utrophin Physiological ...... Body Function in the Canine Model of Duch......ystrophy Restoring Dystrophin Expression by Skipping Exon 6 and 8 in Neonatal Dystr......hic Dogs Restoring Dystrophin Expression wit......44 and 53 Skipping in the DMD Gene in Immortalized Myotubes Restoring Dystrophin Expression wit......y Exon 45 Skipping in Induced-Pluripote......n of Exon Skipping in Urine-Derived Cells......phy Use of Glycine to Augment Exon Skipping and...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2587 Springer protocols (Series)
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc....ree genome editing technologies,......nimal. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Genome Editing in Animals: Methods and Protocols,......er their study in this field
- Contents...Evaluation of Zinc Finger Nucleases U...... SNPD-CRISPR: Single Nucleotide ......lymorphism-Distinguishable Repre......R System Utilizing large functio......by cytoplasmic injection of CRIS......RNA Gene targeting in mouse embryoni......ribonucleoprotein (RNP) mediated genome editing Generation of Knock-in Mouse by Genome Editing Introduction of g......etic mutations into mice by Base......AID Genome editing in mouse and rat ......of flox mice using in vitro Cre recombination VCre/Vlox......specific recombination systems for genome engineering Efficient CRI......-Assisted Knockin of Large DNA D......ronuclear Microinjection During S-phase in Mous...
- Subject HeadingAnimal genetic engineering Transgenic animals Gene editing Genetics--Tec......mal genetic engineering. Gene editing. Genetics--Technique. Transg...
Paper図書
National Diet Library
- Summary, etc....ecent progress in RNA secondary,......an expansive point of view. Beca......f advancements in experimental p......d devices, the integration of ne......ructure-disrupting mutations, hi......hly successful Methods in Molecular Biology series, chapte......s feature the kind of detailed implementation a...
- Contents...l Analyses on Single RNA Sequences Linear-Time Algori......ure Prediction Including Pseudoknots t......ion Caused by Single Nucleotide ......NA Sequences Using TOPAS Fast RNA-RNA Interaction Prediction Methods for Interaction Analy......es for RNA-RNA Interaction Prediction ResidualBind: Uncovering Sequence-Stru......rences of RNA-Binding Proteins with Deep Neu......tructure Determination by High-T......is RNA 3D Modeling with FARFAR2, Online Automated 3D ......e Comparison Using RNA-Puzzles Toolkit
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2586 Springer protocols (Series)
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc....mentation pertaining to chemistry, biology, physiology, p......gy of CCN proteins. The chapters in this book cove......roarray; utilizing public molecular biological dat......says for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithe......nesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp......sive, CCN Proteins: Methods and Protocols,......esearchers looking for new appro......hers just starting out in the field of CCN research
- ContentsCCN Proteins - Cellular Co......Factors: Expanding their Reperto......uorescence Imaging for the Evalu......CN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity......ation Assay of Interaction Betwe......CCN2 and Rab14 in Chondrocytes W......is of CCN Proteins and Fragments in Exosomes, Vesi......of CCN Factors in/on Exosomes Ev......luation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Sur......cols for Screening Peptides Binding to CCN Family Proteins and their Ext......ransfection, Spinfection, Exofection, and Lucif...
- Subject HeadingCysteine--Laboratory manuals Protein-protein interactions--Lab......y manuals Cysteine. Protein-protein interactions.
Paper図書
National Diet Library
- Summary, etc....chnologies and methods--combined with new gen......ools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sectio......ee explores bioinformatics and imaging analysis for ......cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and comp...... Regeneration: Methods and Protocols ......esearchers looking to expand the......egeneration, aging, and regenerative medicine
- Contents...lation, and Staining of Murine Muscle Satell......ell Colony Forming Cell Assay as...... from Regenerating Skeletal Muscle Measuring Oxygen Consum......on Rate (ECAR) in Muscle Stem Cells Using a Seahorse An......cability for Aging Studies High ......roughput Screening of Mitochondr...... Bioenergetics in Myoblasts and ......al Progenitors in Skeletal Muscl......vated Cell Sorting and Tissue Clearing In Vitro Maturati......tem Cells from Induced Pluripote......em Cells Producing Engraftable S......Techniques for Injury, Cell Tran......gical Analysis in Skeletal Muscle Murine Models of Tenotomy-Induced Mechanical Overloading a...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2640 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....e presents cutting-edge research......lity systems using bacterial genetics, molecular biology, biochemistry,......cs, structural biology, cell biology, microscopy imaging, and molecular dynamics simulation. Beginning with a sectio......flagellar protein export and ass......, the book continues with chapters covering flagella-driv......V-driven twitching motility of b......sion-based gliding motility of b......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......esearchers working in this vital area of microbiology
- Contents...cretion System In Vitro Flagella...... Type III Protein Transport Assay Using Inverted Membrane Vesicles Molecular Simulation to Investigate Open-......Apparatus Protein FlhAC Live-Cell Imaging of the Assemb......c Isotope Labeling of FliG for Studying Structural Dynamics Using Nuclear Magne......Directed Crosslinking between Bacte......lar Motor Proteins In Vivo Measureme......Stator Complex in the Bacterial ......Its Analysis Using ATR-FTIR Spec......Complexes and Single-Molecule Imaging by High-Speed......r Zero Loads Using a Mutant Having a Rod-Like St......uorescence Imaging of Magnetosom...... Motility Swarming Motility Assays in Salmonell...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2646 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....ogies on recombinant DNA and nuc......ion that underpin much of the bi......l sciences and instructions on h......and DNA sequencing; recombinant DNA plasmid......t developments in CRISPR approac......ation. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... materials and methods, includes tips on troubleshooting and known pit......tative and cutting-edge, DNA Man...... scientists moving into the field of recombinant DNA and nucleic acid manip...
- ContentsClassical recombinant DNA cloning / Ana Mikić,......- and ligation-independent cloning (SLIC) procedure for the insertion of genes into a plasmid ve......ert A. Holland Molecular cloning using in vivo DNA assem......Nafría Assembling multiple frag...... Avilan TA cloning approaches to cloning DNA with dama....../ Charlotte Ayling-- PCR-based a......cally balanced inside-out (TBIO)...... and Helen A. Vincent Random mut...... / F. A. Myers In vitro site dir......transgenesis using the pGateway ......uption studies in F0 Xenopus tad......les: understanding development and disease in the frog / Ani...... and Annie Godwin A CRISPR/Cas based method for...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2633 Springer protocols (Series)
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc.This volume provides methods on different a...... multiplex cloning. Chapters cov......olic and signaling pathways, advanced engineering of metabolic ......organisms, and methods for the on com......bility of proteins. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Plant Synthetic Biology: Methods and Protocols ......searchers starting to explore no......or experts willing to expand their portfolio of...
- Contents...plant terpenes in the photosynth......gn of multiplexing CRISPR/Cas9 c......lant genome engineering using the GoldenBra......for genetic engineering of cyanobacte......ed mutagenesis in plants Generat......robes Gene editing in green alga Chlamydomonas reinhardtii via CRI......ribonucleoproteins Coexpression ......matic cascades in bacteria using UbiGate Homo-FRET imaging to study protein-protein interaction and c......plex formation in plants Plant X......iptional units in plants AGROBES......ression system in Arabidopsis seedlings Genetically-......analysis of auxin dynamics in plant cells Exploiting the Gal4/UAS system as plant...
- Subject Heading...tion Synthetic Biology Biologie synth......logy Synthetic biology
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc.... plant systems biology on three speci......ic scales: the molecular level, the tis......latory network inference and dynamic modeling using ordinary differentia......ls for at-will induction of plan......hematical modeling of plant tissue using SimuPlant or ......af; and simulating crop root systems using OpenSimRoot o......-SWMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......ensive and cutting-edge, Plants Systems Biology: Methods and Protocols,......biologists looking for different......approaches to finding solutions to their questi...
- ContentsPlant Systems Biology: Lessons from Teaching How to Use th......r Algorithm to Infer Gene Regula......latory Network Investigation Using Ordinary Differentia...... Organogenesis in Plants: Experimental Induction of New ......oot Organs NanoIndentation, an ImageJ Plugin for the Quanti......Cell Mechanics Introduction to C......utational Modeling of Multicellu......ematical Modelling of Auxin Transport in the Arabidopsis Root Tip using the Open Sour......SimuPlant Modeling Plant Tissue Development using VirtualLeaf Identifying Developmental Patterns in Structured Plant Phenotyping Data Putting Plant Roots a......: Temporal Imaging of Plant Roots and Soil Wate...
- Subject HeadingPlant molecular biology Systems biology Systems Biology Biologie moléculaire végét...
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc....te-of-the- art methods on computer-ai......eaders through information on an......ructures, modeling antibody stru......tibody-antigen interactions, and......ed antibody affinity maturation ......eyond. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Compute......nd experts looking to expand their knowledge
- Contents...ure analyses using IMGTª: 30 years of immunoinformatics Struc......tion of CDR-H3 in Single-Domain VHH Antibodies......tational modelling of Antibody a......r (CDR3 loops) Molecular dynamics simulation for investigating antigen-antibody interaction Molecular Dynamics Methods for Antibody Design Probing antibody dyna......Estimation of Single Domain Antibodies Using Molecular Dynamics Simulations Assessing and engineering antibody stability using experimental ...... computational methods In Silico Predict......ethod for Protein Asparagine Deamidation S......ical Guide for Molecular Modelers B-cel...... predictions using computational methods Comput...
- Subject HeadingImmunoinformatics Immunoglobulins--Computer-aided design Immunoinformatics.
Paper図書
National Diet LibraryOther Libraries in Japan
- Summary, etc....ition with new information on th......sis of glycosaminoglycans (GAGs)......is of GAG-protein interactions; molecular and genetic ap......es to manipulating GAG cell biology; and molecular and genetic methods involving animal models......me also offers insight and guida......who are performing advanced expe...... GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and auth......ative, Glycosaminoglycans: Methods and Protocols,......ual for practicing researchers including graduate and post-doct...
- Subject HeadingGlycosaminoglycans--Laboratory manuals Glycosaminoglycans.
- Note (General)Includes index
Paper図書
National Diet Library
- Summary, etc....of hepatocytes in vivo and in vitro, and for characterizing heterogeneity......of hepatocytes in healthy and in injured liver. Ch......through separating hepatocyte su......ns, characterizing the heterogen......m cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepat......ma and identifying hepatocellular carcinoma subtypes. Written in the format of ......hly successful Methods in Molecular Biology series, each chapter includes an introduction to t...... and reagents, includes tips on troubleshooting and known pit......tative and cutting-edge, Hepatocytes: Methods a...
- Contents... Generation of in vivo traceable......uman iPS cells Induction of bile......canaliculi forming hepatocytes from human induced pluripote......psulated small interfering RNA Co-cultur......atitis B virus infection using iPSC-derived ......lls and liver sinusoidal endothelial cells In vitro assay sy......to detect drug-induced bile acid......cytotoxicity using hepatocytes E......lux analysis using mass spectrom...... Live Cell Imaging of Biosynthetic Protein Transport in Hepatocytes Co......or image-based investigations.-A......ls from retrorsine/partial hepat...... proliferation in situ during liver regeneration Quantific...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2544 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....sections detailing modification ......f microtubules in vitro, develop......f microtubule-binding molecules, co......f microtubules in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Microtubules: Methods and Protocols ......provide useful information for construction of ...
- ContentsPurification of Tubulin from Porcine Brain and its Fluore......Method of Tubulin Purification from Goat Brain for Reconstitu......ule Associated Intracellular Fun......zation of Tubulin: Approaches to Modify Tubulin with Biotin and DNA Electr......lation of Tubulin Properties and Function In Vitro Reconsti......amics and Severing Imaged by Label-Free Interference Refl......opy Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microsco...... Microtubules Linear-Zero Mode Waveguides for Single-Molecule Fl......of Nucleotides in Kinesin-Microtubule Mo......d Quantum Dots Integration Leads to Conjugates ...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2430 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....s applications in molecular biological tec......tions to determine the involvement of glycans in virus interactions. Cha......glycan and lectin microarray, pr......ation of recombinant viral domain protein, reverse genet...... and receptor binding, virus-host interactions and receptor binding, and sialidase: assays and inhibitors. Written in the successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfall......tative and cutting-edge, Glycovirology: Methods and Protocols aims to be an e...
- Contents...r glycan profiling of glycoproteins and glycosphingolipids by glycoblotting and BEP methods High-performan......matography mapping method for th......glycans at the molecular, cellular, and......cell surface using flow cytometry Lectin Staining to Detect Human and Avian influenza Virus Receptors in the Airway of ......lycan Arrays Using Glycopeptides......s Glycan profiling of viral glycoproteins with the lectin microarray Pre......ation of recombinant PA endonuclease domain protein of influenza A virus......ation for glycobiology research Receptor-binding specificity of influenza viruses...... of sulphatide in influenza A virus infection a...
- Title HeadingMethods in molecular biology (Clifton, N.J.) ; v. 2556 Springer protocols (Series)
Paper図書
National Diet Library
- Summary, etc....ewly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently appl......o other ncRNAs involved in nuclear regula......NAs, and piRNA methods applied in non-classical ......nisms. It also includes several bioinformatic and biophysical methods related to piR......stent with the increasing importance of......oughput sequencing and computational methods. Written for t......hly successful Methods in Molecular Biology series, chapters include introductions to ......on troubleshooting and avoiding known pitfall......o-date, piRNA: Methods and Protocols ......esearchers seeking to elucidate the numerous my...
- ContentsFunctional Analysis of Individual piRNAs in Aedes aegypti ...... and Embryos Using Antisense Oli......ated Genome Engineering in Aedes aegypti ......rected DNA Elimination for Tetra......ian PIWI-piRNA Interaction Analysis Using Immunoprecipi......d piRNA-Sequencing Isolation and Processing of Bovine Oocytes for S......all RNA Sequencing 3D Imaging and In Situ Hybridiza......ion for Uncovering the Functions of MicroRNA in Rice Anther Cloning, Sequencing, and Linkage Analysis o......es for Elucidating piRNA Pathway...... Somatic Cell Lines Using the piggyBac ......t RNA FISH Combined with Immunof......Ribonucleoproteins in the Drosophila Germline C...
- Subject HeadingSmall interfering RNA Small interfering RNA.
Paper図書
National Diet Library
- Summary, etc....techniques and methods used for performing up-to-date gl....... The chapters in this book are organized into four parts. ......lytical and bioinformatics techn......ry and glycoengineering in the fields of bioprocessing and biotherap......cusses systems biology and computatio......ylation events in the cell. Part......terize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to ......on troubleshooting and avoiding known pitfalls. Cutting-edge and prac......Glycosylation: Methods and Protocols ...... or researcher interested in learning more abou...
- ContentsGlycoinformatics Tools......ased from Proteins GlycoStore: A......Glycan Data An Interactive View ......ll Free Remodeling of Glycosylat......tibody Glycoengineering in CHO Cells via RNA Interference and ......IF N-Glycomics In Silico Analysi......gation and the Influence of Glyc......ces Towards Engineering Glycoproteins using Modified Yeas......utational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangemen......ectrometry Lectin Histochemistry......, and Dual Lectin and Antibody C......calization Lectin Affinity Chromatogra......etabolic Labelling of Primary Neurons using Carbohydrate Click-Chemistry...
- Subject HeadingGlycosylation Molecular biology Molecular Biology Biologie moléculaire molecular biology. Glycosylation. Molecular biology.