Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol
デジタルデータあり（科学技術振興機構）
すぐに読む
J-STAGE
全国の図書館の所蔵
国立国会図書館以外の全国の図書館の所蔵状況を表示します。
所蔵のある図書館から取寄せることが可能かなど、資料の利用方法は、ご自身が利用されるお近くの図書館へご相談ください
その他
J-STAGE
デジタルCiNii Research
検索サービスデジタル連携先のサイトで、CiNii Researchが連携している機関・データベースの所蔵状況を確認できます。
書誌情報
この資料の詳細や典拠（同じ主題の資料を指すキーワード、著者名）等を確認できます。
- 資料種別
- 記事
- 著者・編者
- Takahito ABERyouka KAWAHARA-MIKITomotaka HARATatsuo NOGUCHITakeshi HAYASHIKoumei SHIRASUNATakehito KUWAYAMAHisataka IWATA
- 著者標目
- タイトル（掲載誌）
- The journal of reproduction and development : official journal of the Society for Reproduction and Development
- 巻号年月日等（掲載誌）
- 63(5):2017.10
- 掲載巻
- 63
- 掲載号
- 5
- 掲載ページ
- 455-461
- 掲載年月日（W3CDTF）
- 2017-10
- ISSN（掲載誌）
- 0916-8818
- ISSN-L（掲載誌）
- 0916-8818
- 出版事項（掲載誌）
- Tokyo : Society for Reproduction and Development
- 出版地（国名コード）
- JP
- 本文の言語コード
- eng
- NDLC
- 対象利用者
- 一般
- 所蔵機関
- 国立国会図書館
- 請求記号
- Z54-H305
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- 書誌ID（NDLBibID）
- 028582425
- 整理区分コード
- 632
- 要約等
- <p> Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism and ATP generation in somatic cells. In the present study, the effects of supplementing culture medium with resveratrol on lipid metabolism, ATP generation, and cryosensitivity of bovine <i>in vitro</i> produced embryos were investigated. Bovine early cleaved-stage embryos were cultured in medium containing 0 or 0.5 µM resveratrol for 1 or 5 days. Resveratrol treatment for both 1 day and 5 days increased the expression levels of SIRT1 and phosphorylated AMP-activated protein kinase (pAMPK) in the embryos. Furthermore, resveratrol treatment was effective to increase ATP generation and reduce lipid content of the embryos. The effects of resveratrol treatment were diminished by the SIRT1 inhibitor “EX527”, and the reduced lipid content was reversed by treatment with etomoxir (a potent inhibitor of beta-oxidation). Blastocysts developed after resveratrol treatment showed low levels reactive oxygen species and increased cryotolerance. These results demonstrate that resveratrol improves <i>in vitro</i> development of bovine embryos, while reducing cytoplasmic lipid content through activation of beta-oxidation, thereby effective for production of bovine blastocysts with enhanced cryotolerance.</p>
- DOI
- 10.1262/jrd.2016-182
- オンライン閲覧公開範囲
- インターネット公開
- 連携機関・データベース
- 科学技術振興機構 : J-STAGE
- 要約等
- <p> Resveratrol is a potent activator of NAD-dependent deacetyltransferase sirtuin-1 (SIRT1) and affects lipid metabolism and ATP generation in somatic cells. In the present study, the effects of supplementing culture medium with resveratrol on lipid metabolism, ATP generation, and cryosensitivity of bovine <i>in vitro</i> produced embryos were investigated. Bovine early cleaved-stage embryos were cultured in medium containing 0 or 0.5 µM resveratrol for 1 or 5 days. Resveratrol treatment for both 1 day and 5 days increased the expression levels of SIRT1 and phosphorylated AMP-activated protein kinase (pAMPK) in the embryos. Furthermore, resveratrol treatment was effective to increase ATP generation and reduce lipid content of the embryos. The effects of resveratrol treatment were diminished by the SIRT1 inhibitor “EX527”, and the reduced lipid content was reversed by treatment with etomoxir (a potent inhibitor of beta-oxidation). Blastocysts developed after resveratrol treatment showed low levels reactive oxygen species and increased cryotolerance. These results demonstrate that resveratrol improves <i>in vitro</i> development of bovine embryos, while reducing cytoplasmic lipid content through activation of beta-oxidation, thereby effective for production of bovine blastocysts with enhanced cryotolerance.</p>
- DOI
- 10.1262/jrd.2016-182
- オンライン閲覧公開範囲
- インターネット公開
- 関連情報（URI）
- 参照
- Resveratrol enhances the clearance of mitochondrial damage by vitrification and improves the development of vitrified-warmed bovine embryosRescue of vitrified-warmed bovine mature oocytes by short-term recovery culture with resveratrolLycium barbarum polysaccharide enhances development of previously-cryopreserved murine two-cell embryos via restoration of mitochondrial function and down-regulated generation of reactive oxygen species
- 参照
- Effect of 5-aminoimidazole-4-carboxamide ribonucleoside on the mitochondrial function and developmental ability of bovine oocytesCryosurvival of bovine blastocysts is enhanced by culture with trans-10 cis-12 conjugated linoleic acid (10t,12c CLA)Improved survival of vitrified porcine embryos after partial delipation through chemically stimulated lipolysis and inhibition of apoptosisInfluence of Delipation on the Energy Metabolism in Pig Parthenogenetically Activated EmbryosSIRT1 Is Required for AMPK Activation and the Beneficial Effects of Resveratrol on Mitochondrial FunctionResveratrol improves the quality of pig oocytes derived from early antral follicles through sirtuin 1 activationRelationship between mitochondrial DNA Copy Number and SIRT1 Expression in Porcine OocytesEmerging beneficial roles of sirtuins in heart failureBeneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilizationIn vivo versus in vitro produced bovine ova: similarities and differences relevant for practical applicationRemoval of Cytoplasmic Lipid Enhances the Tolerance of Porcine Embryos to ChillingEffects of follicle size and electrolytes and glucose in maturation medium on nuclear maturation and developmental competence of bovine oocytesFertilizability and developmental capacity of individually cultured bovine oocytesTrichostatin A-treated eight-cell bovine embryos had increased histone acetylation and gene expression, with increased cell numbers at the blastocyst stageExpression patterns of sirtuin genes in porcine preimplantation embryos and effects of sirtuin inhibitors on in vitro embryonic development after parthenogenetic activation and in vitro fertilizationAMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activityIn vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitaminsSet up of a serum-free culture system for bovine embryos: Embryo development and quality before and after transient transferSIRT1 modulates MAPK pathways in ischemic–reperfused cardiomyocytes<scp>AMP</scp>‐activated protein kinase: a key regulator of energy balance with many roles in human diseaseEnergy Metabolism in Preimplantation Bovine Embryos Derived In Vitro or In Vivo1Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytesLipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrificationCryopreservation of porcine embryosEffect of resveratrol supplementation during culture on the quality and cryotolerance of bovine in vitro produced embryosFatty acid oxidation and meiotic resumption in mouse oocytesCryotolerance of Bovine Blastocysts is Affected by Oocyte Maturation in Media Containing Palmitic or Stearic AcidCorrection: Amino acid metabolism of bovine blastocysts: A biomarker of sex and viabilityEvaluation of Bovine Embryos Produced in High Performance Serum-Free MediaEffect of Maternal Age on the Ratio of Cleavage and Mitochondrial DNA Copy Number in Early Developmental Stage Bovine EmbryosResveratrol Improves the Mitochondrial Function and Fertilization Outcome of Bovine OocytesEffect of Resveratrol on the Development of Porcine Embryos Produced In VitroDevelopmental ability of porcine in vitro matured oocytes at the meiosis 2 stage after vitrification
- 連携機関・データベース
- 国立情報学研究所 : CiNii Research
- 提供元機関・データベース
- Japan Link Center雑誌記事索引データベースCrossrefPubMedCiNii ArticlesCrossrefCrossrefCrossref
- 書誌ID（NDLBibID）
- 028582425
- NII論文ID
- 130006172810