Assessment of spawning events of the tri-spine horseshoe crab, Tachypleus tridentatus, using environmental DNA in the Sone tidal flat, Japan
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- 資料種別
- 記事
- 著者・編者
- AKIHIKO KOYAMAYUKI KAWAHARAYOSHIHISA KURITATAIGA HIRATAOSAMU HAYASHISHUNGO TAKAHASHIHARUKA KUBOZONONORIO ONIKURATOMOKO MINAGAWA
- 著者標目
- タイトル（掲載誌）
- Plankton & benthos research : a joint journal of the Plankton Society of Japan & the Japanese Association of Benthology
- 巻号年月日等（掲載誌）
- 17(2):2022.5
- 掲載巻
- 17
- 掲載号
- 2
- 掲載ページ
- 127-136
- 掲載年月日（W3CDTF）
- 2022-05
- ISSN（掲載誌）
- 1880-8247
- ISSN-L（掲載誌）
- 1880-8247
- 出版事項（掲載誌）
- [Hakodate] : Plankton Society of Japan ; 2006-
- 出版地（国名コード）
- JP
- 本文の言語コード
- eng
- NDLC
- 対象利用者
- 一般
- 所蔵機関
- 国立国会図書館
- 請求記号
- Z54-J424
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- 書誌ID（NDLBibID）
- 032186260
- 整理区分コード
- 632
- 要約等
- <p>The conservation of an endangered horseshoe crab, <i>Tachypleus tridentatus</i>, has been increasingly emphasized worldwide. Although monitoring of <i>T. tridentatus</i> reproduction is effective for its conservation, the conventional monitoring system for spawning has several drawbacks. Thus, our study aimed to examine the environmental DNA (i.e., eDNA) approach to assess <i>T. tridentatus</i> spawning events. Six survey sites were set at the sandy beaches of the Sone tidal flat, Japan. Visual observations of the spawning events, i.e., the presence/absence of adult pairs of <i>T. tridentatus</i> or that of foams, occurring because of their spawning behavior, and water sampling for eDNA assay were conducted 10 times at each site between 2019 and 2020. The spawning events were observed at a total 16 sites during the surveys, and the estimated numbers of <i>T. tridentatus</i> adult pairs were 1–5 per site. <i>T. tridentatus</i> DNA was detected in 15 of the 16 samples at the sites where the spawning events were observed and in 13 of 44 samples where the spawning events were not observed. The estimated number of pairs and the eDNA concentration showed a significantly positive correlation. Moreover, the presence/absence of the spawning events was distinguished with excellent accuracy based on the eDNA concentration. Therefore, our results showed that the spawning events of the species and the eDNA concentration were closely related. In conclusion, we expect that the population of <i>T. tridentatus</i> in spawning grounds can be evaluated more complementarily by combining the conventional observation method with the eDNA approach.</p>
- DOI
- 10.3800/pbr.17.127
- オンライン閲覧公開範囲
- インターネット公開
- 連携機関・データベース
- 科学技術振興機構 : J-STAGE
- 要約等
- <p>The conservation of an endangered horseshoe crab, <i>Tachypleus tridentatus</i>, has been increasingly emphasized worldwide. Although monitoring of <i>T. tridentatus</i> reproduction is effective for its conservation, the conventional monitoring system for spawning has several drawbacks. Thus, our study aimed to examine the environmental DNA (i.e., eDNA) approach to assess <i>T. tridentatus</i> spawning events. Six survey sites were set at the sandy beaches of the Sone tidal flat, Japan. Visual observations of the spawning events, i.e., the presence/absence of adult pairs of <i>T. tridentatus</i> or that of foams, occurring because of their spawning behavior, and water sampling for eDNA assay were conducted 10 times at each site between 2019 and 2020. The spawning events were observed at a total 16 sites during the surveys, and the estimated numbers of <i>T. tridentatus</i> adult pairs were 1–5 per site. <i>T. tridentatus</i> DNA was detected in 15 of the 16 samples at the sites where the spawning events were observed and in 13 of 44 samples where the spawning events were not observed. The estimated number of pairs and the eDNA concentration showed a significantly positive correlation. Moreover, the presence/absence of the spawning events was distinguished with excellent accuracy based on the eDNA concentration. Therefore, our results showed that the spawning events of the species and the eDNA concentration were closely related. In conclusion, we expect that the population of <i>T. tridentatus</i> in spawning grounds can be evaluated more complementarily by combining the conventional observation method with the eDNA approach.</p>
- DOI
- 10.3800/pbr.17.127
- オンライン閲覧公開範囲
- インターネット公開
- 関連情報（URI）
- 参照
- Morphological allometry and life history including reproductive swarming of nereidid polychaete Tylorrhynchus osawai in a tidal river in Kyushu, Japan日本温帯河口・沿岸における潮間帯生息場の評価における課題
- 参照
- An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocolsHabitat suitability maps for juvenile tri-spine horseshoe crabs in Japanese intertidal zones: A model approach using unmanned aerial vehicles and the Structure from Motion techniqueEnvironmental <scp>DNA</scp> analysis for estimating the abundance and biomass of stream fishA novel environmental <scp>DNA</scp> approach to quantify the cryptic invasion of non‐native genotypesREVIEW: The detection of aquatic animal species using environmental DNA – a review of eDNA as a survey tool in ecologyRapid Decline of Nassau Grouper Spawning Aggregations in Belize: Fishery Management and Conservation NeedsMonitoring of noble, signal and narrow-clawed crayfish using environmental DNA from freshwater samplesReproductive Competition and Sexual Selection in Horseshoe CrabsIdentifying spawning events in fish by observing a spike in environmental DNA concentration after spawningAn environmental <scp>DNA‐</scp>based method for monitoring spawning activity: a case study, using the endangered Macquarie perch (<i>Macquaria australasica</i>)A review on fisheries and conservation status of Asian horseshoe crabsBasic Habitat Requirements of the Extant Species of Horseshoe Crabs (Limulacea)Conservation Program for the Asian Horseshoe Crab Tachypleus tridentatus in Taiwan: Characterizing the Microhabitat of Nursery Grounds and Restoring Spawning GroundsEnvironmental DNA shedding and decay rates from diverse animal forms and thermal regimesHorseshoe Crab Research in Urban Estuaries: Challenges and OpportunitiesCitizen science frontiers horseshoe crab population regain at their spawning beach in East Peninsular MalaysiaPublic Awareness and Community-Based Conservation for the Horseshoe Crab at Saikai National Park in Nagasaki Prefecture, JapanSpatial and temporal distribution of horseshoe crab (Limulus polyphemus) spawning in Delaware Bay: Implications for monitoringFuture of Asian horseshoe crab conservation under explicit baseline gaps: A global perspectiveSave horseshoe crabs and coastal ecosystemsSignals from the benthos: Development and evaluation of a benthic index for the nearshore Gulf of MaineAdaptive management of an environmental watering event to enhance native fish spawning and recruitmentMonitoring endangered freshwater biodiversity using environmental DNACan environmental DNA (eDNA) be used for detection and monitoring of introduced crab species in the Baltic Sea?Conservation in a cup of water: estimating biodiversity and population abundance from environmental DNAPresent and Potential Future Distributions of Asian Horseshoe Crabs Determine Areas for ConservationEnvironmental<scp>DNA</scp>surveillance for invertebrate species: advantages and technical limitations to detect invasive crayfish<i><scp>P</scp>rocambarus clarkii</i>in freshwater pondsThe Measurement of Observer Agreement for Categorical DataEnvironmental DNA as a non‐invasive sampling tool to detect the spawning distribution of European anadromous shads (<i>Alosa</i> spp.)A comparison of droplet digital polymerase chain reaction (PCR), quantitative PCR and metabarcoding for species‐specific detection in environmental DNAUsing environmental DNA methods to improve detectability in a hellbender (Cryptobranchus alleganiensis) monitoring programDeveloping an eDNA toolkit to quantify broadcast spawning events of the sea scallop Placopecten magellanicus: moving beyond fertilization assaysConserving the understudied invertebrates: a call for a systematic monitoring protocol for Asian horseshoe crabs in nursery habitatsConservation of the horseshoe crab at Kinmen, Taiwan: strategies and practicesIndex for rating diagnostic testsApplied Logistic RegressionApplications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospectsUsing environmental DNA analyses to assess the occurrence and abundance of the endangered amphidromous fish Plecoglossus altivelis ryukyuensisUsing eDNA to develop a national citizen science-based monitoring programme for the great crested newt (Triturus cristatus)Environmental DNA (eDNA) detects the invasive crayfishes Orconectes rusticus and Pacifastacus leniusculus in large lakes of North America曽根干潟におけるカブトガニ幼生生息環境の物理特性に関する基礎的研究
- 連携機関・データベース
- 国立情報学研究所 : CiNii Research
- 提供元機関・データベース
- Japan Link Center雑誌記事索引データベースCrossrefCrossrefCrossref
- 書誌ID（NDLBibID）
- 032186260