実験的構造解析とAI構造予測が解明する卵子細胞外マトリックスの動的構造変化 : 多精子受精拒否の分子機構(ミニ特集 AIがもたらす構造生物学研究の変革)

• 資料種別: 記事
• タイトル: 実験的構造解析とAI構造予測が解明する卵子細胞外マトリックスの動的構造変化 : 多精子受精拒否の分子機構
• 著者・編者: 西尾 俊亮
• シリーズタイトル: ミニ特集 AIがもたらす構造生物学研究の変革
• 著者標目: 西尾 俊亮
• 並列タイトル等: Experimental and AI-Predicted Structural Analysis Reveals Dynamic Changes in Oocyte Extracellular Matrix : Molecular Mechanisms of Polyspermy Block
• タイトル（掲載誌）: 日本結晶学会誌 = Journal of the Crystallographic Society of Japan
• 巻号年月日等（掲載誌）: 67(3):2025.8
• 掲載巻: 67
• 掲載号: 3
• 掲載ページ: 189-195
• 掲載年月日（W3CDTF）: 2025-08
• ISSN（掲載誌）: 0369-4585
• ISSN-L（掲載誌）: 0369-4585
• 出版事項（掲載誌）: 東京 : 日本結晶学会
• 出版地（国名コード）: JP
• 本文の言語コード: jpn
• NDLC: ZM46
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z15-138
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 034356263; http://id.ndl.go.jp/bib/034356263
• 整理区分コード: 632

• 要約等: <p>Fertilization is the process by which male and female gametes fuse to form a fertilized egg. In mammals and amphibians, polyspermy is lethal. Upon fertilization, egg-derived proteases specifically cleave ZP2, one of the glycoproteins surrounding the oocyte, associated with egg coat hardening. However, the molecular mechanisms and egg coat structure remained unknown. We combined X-ray crystallography, cryo-electron microscopy, and AlphaFold2 structure prediction to elucidate the three-dimensional structure of egg coat matrix formed by ZP glycoprotein polymerization and the molecular mechanisms underlying polyspermy block through egg coat structural changes.</p>
• DOI: 10.5940/jcrsj.67.189
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: <p>Fertilization is the process by which male and female gametes fuse to form a fertilized egg. In mammals and amphibians, polyspermy is lethal. Upon fertilization, egg-derived proteases specifically cleave ZP2, one of the glycoproteins surrounding the oocyte, associated with egg coat hardening. However, the molecular mechanisms and egg coat structure remained unknown. We combined X-ray crystallography, cryo-electron microscopy, and AlphaFold2 structure prediction to elucidate the three-dimensional structure of egg coat matrix formed by ZP glycoprotein polymerization and the molecular mechanisms underlying polyspermy block through egg coat structural changes.</p>
• DOI: 10.5940/jcrsj.67.189; https://doi.org/10.5940/jcrsj.67.189
• 関連情報（URI）: http://id.ndl.go.jp/bib/034356263; https://ndlsearch.ndl.go.jp/books/R000000004-I034356263; https://www.jstage.jst.go.jp/article/jcrsj/67/3/67_189/_pdf
• 参照: Highly accurate protein structure prediction with AlphaFold; https://cir.nii.ac.jp/crid/1360292274168292992; Three-dimensional structure of the zona pellucida; https://cir.nii.ac.jp/crid/1360306505359691520; Insights into Egg Coat Assembly and Egg-Sperm Interaction from the X-Ray Structure of Full-Length ZP3; https://cir.nii.ac.jp/crid/1360565165805694592; Cryo‐EM structure of native human uromodulin, a zona pellucida module polymer; https://cir.nii.ac.jp/crid/1360853567535164800; ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat; https://cir.nii.ac.jp/crid/1360865816798520704; Gamete Recognition in Mice Depends on the Cleavage Status of an Egg’s Zona Pellucida Protein; https://cir.nii.ac.jp/crid/1361137043492344064; Cell surface changes in the egg at fertilization; https://cir.nii.ac.jp/crid/1361137043766179072; Mouse egg extracellular coat is a matrix of interconnected filaments possessing a structural repeat; https://cir.nii.ac.jp/crid/1361418520631986560; <i>Xenopus laevis</i> sperm receptor gp69/64 glycoprotein is a homolog of the mammalian sperm receptor ZP2; https://cir.nii.ac.jp/crid/1361418520704542848; Polyspermy barriers: a plant perspective; https://cir.nii.ac.jp/crid/1362262946043332864; Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy; https://cir.nii.ac.jp/crid/1363951796068371072
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org
• 書誌ID（NDLBibID）: 034356263; http://id.ndl.go.jp/bib/034356263