高精度1分子・超解像顕微鏡観察による細胞外小胞の膜動態の解明(特集 特別講演を基調とした総説)

• 資料種別: 記事
• タイトル: 高精度1分子・超解像顕微鏡観察による細胞外小胞の膜動態の解明
• 著者・編者: 鈴木 健一
• シリーズタイトル: 特集 特別講演を基調とした総説
• 著者標目: 鈴木 健一
• タイトル（掲載誌）: 膜 = Membrane / 膜編集委員会 編
• 巻号年月日等（掲載誌）: 50(6)=300:2025.11
• 掲載巻: 50
• 掲載号: 6
• 掲載通号: 300
• 掲載ページ: 242-247
• 掲載年月日（W3CDTF）: 2025-11
• ISSN（掲載誌）: 0385-1036
• ISSN-L（掲載誌）: 0385-1036
• 出版事項（掲載誌）: 東京 : 日本膜学会
• 出版地（国名コード）: JP
• 本文の言語コード: jpn
• 件名標目: extracellular vesicles; internalization; single particle tracking; high-speed super-resolution microscopy
• NDLC: ZR2
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z18-1127
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 034459909; http://id.ndl.go.jp/bib/034459909
• 整理区分コード: 632

• 要約等: Extracellular vesicles (EVs) play critical roles in intercellular communications. Recent studies suggested that tumor–derived EVs induce phenotypic changes in target cells, creating a premetastatic niche that promotes cancer metastasis. Here, I introduce studies on the interaction between EVs and target cells by single–particle tracking and super–resolution movie observation. EVs could be categorized into several subtypes, which have different tetraspanin marker proteins. Integrin α6β4 and α6β1, with the aid of CD151 in all the EV subtypes, bound to laminin more frequently than fibronectin on the target cell surface. EV subtypes containing CD63 have lower membrane fluidity and induce the accumulation of raft–like structures underneath the EV in the target cell membrane. Upon the EV binding, integrin β1 molecules are accumulated underneath the EVs in the target cell membrane, which trigger intracellular signal transduction, Ca2+ mobilization, activation of dynamin, and promote internalization of the EVs by target cells. Thus, this review introduces the studies on dynamics of EVs and their association with target cells as revealed by high–resolution imaging.
• DOI: 10.5360/membrane.50.242
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Extracellular vesicles (EVs) play critical roles in intercellular communications. Recent studies suggested that tumor–derived EVs induce phenotypic changes in target cells, creating a premetastatic niche that promotes cancer metastasis. Here, I introduce studies on the interaction between EVs and target cells by single–particle tracking and super–resolution movie observation. EVs could be categorized into several subtypes, which have different tetraspanin marker proteins. Integrin α6β4 and α6β1, with the aid of CD151 in all the EV subtypes, bound to laminin more frequently than fibronectin on the target cell surface. EV subtypes containing CD63 have lower membrane fluidity and induce the accumulation of raft–like structures underneath the EV in the target cell membrane. Upon the EV binding, integrin β1 molecules are accumulated underneath the EVs in the target cell membrane, which trigger intracellular signal transduction, Ca2+ mobilization, activation of dynamin, and promote internalization of the EVs by target cells. Thus, this review introduces the studies on dynamics of EVs and their association with target cells as revealed by high–resolution imaging.
• DOI: 10.5360/membrane.50.242; https://doi.org/10.5360/membrane.50.242
• 関連情報（URI）: http://id.ndl.go.jp/bib/034459909; https://ndlsearch.ndl.go.jp/books/R000000004-I034459909; https://www.jstage.jst.go.jp/article/membrane/50/6/50_242/_pdf; https://search.jamas.or.jp/link/ui/2026083349
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org
• 書誌ID（NDLBibID）: 034459909; http://id.ndl.go.jp/bib/034459909