Promoter Sequences Do Not Solely Govern nosZ Expression Differences between Bradyrhizobium ottawaense and B. diazoefficiens(Special issue : Cool Earth via Microbes)

• 資料種別: 記事
• タイトル: Promoter Sequences Do Not Solely Govern nosZ Expression Differences between Bradyrhizobium ottawaense and B. diazoefficiens
• 著者・編者: SAWA WASAI-HARA; YOSHIKAZU SHIMODA; HISAYUKI MITSUI; SHUSEI SATO; HARUKO IMAIZUMI-ANRAKU; KIWAMU MINAMISAWA
• シリーズタイトル: Special issue : Cool Earth via Microbes
• 著者標目: SAWA WASAI-HARA; YOSHIKAZU SHIMODA; HISAYUKI MITSUI; SHUSEI SATO; HARUKO IMAIZUMI-ANRAKU; KIWAMU MINAMISAWA
• タイトル（掲載誌）: Microbes and environments
• 巻号年月日等（掲載誌）: 41(1):2026.3
• 掲載巻: 41
• 掲載号: 1
• 掲載ページ: 1/5-5/5
• 掲載年月日（W3CDTF）: 2026-03
• ISSN（掲載誌）: 1342-6311
• ISSN-L（掲載誌）: 1342-6311
• 出版事項（掲載誌）: Tsukuba : Japanese Society of Microbial Ecology ; 2001-
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• 件名標目: N₂O reduction; greenhouse gases; gene expression; rhizobia; promoter sequence
• NDLC: ZR5
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z54-J644
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 034654841; http://id.ndl.go.jp/bib/034654841
• 整理区分コード: 632

• 要約等: <p>Nitrous oxide (N₂O) is a potent greenhouse gas, and the enzyme Nos catalyzes its reduction to dinitrogen (N₂). <i>Bradyrhizobium ottawaense</i> exhibits strong N₂O-reducing activity with high <i>nosZ</i> expression. To investigate whether promoter sequences affect <i>nosZ</i> expression, we constructed reciprocal promoter-swapped mutants between <i>B. ottawaense</i> and <i>B. diazoefficiens</i>. The swapping of promoters did not significantly affect expression levels. <i>B. ottawaense</i> mutants maintained approximately 200-fold higher expression levels than <i>B. diazoefficiens</i>, and the introduction of the <i>B. ottawaense</i> promoter into <i>B. diazoefficiens</i> did not increase expression levels. Therefore, the present results indicate that promoter sequence differences are not the primary factor affecting <i>nosZ</i> expression, suggesting regulation by other factors.</p>
• DOI: 10.1264/jsme2.me25079; https://doi.org/10.1264/jsme2.me25079
• 関連情報（URI）: http://id.ndl.go.jp/bib/034654841; https://ndlsearch.ndl.go.jp/books/R000000004-I034654841; https://www.jstage.jst.go.jp/article/jsme2/41/1/41_ME25079/_pdf
• 参照: Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils; https://cir.nii.ac.jp/crid/1360011144303981056; Bradyrhizobium ottawaense efficiently reduces nitrous oxide through high nosZ gene expression; https://cir.nii.ac.jp/crid/1360022306717335680; Unlocking bacterial potential to reduce farmland N2O emissions; https://cir.nii.ac.jp/crid/1360023719116107264; Deep Learning‐Assisted Design of Novel Promoters in <i>Escherichia coli</i>; https://cir.nii.ac.jp/crid/1360026343965976832; Mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation; https://cir.nii.ac.jp/crid/1360283691778532480; The nitrate‐sensing <scp>N</scp> as <scp>ST</scp> system regulates nitrous oxide reductase and periplasmic nitrate reductase in <scp> <i>B</i> </scp> <i>radyrhizobium japonicum</i>; https://cir.nii.ac.jp/crid/1360285710425683840; A novel bacterial protein family that catalyses nitrous oxide reduction; https://cir.nii.ac.jp/crid/1360307817250104576; Systematic modulation of bacterial resource allocation by perturbing RNA polymerase availability via synthetic transcriptional switches; https://cir.nii.ac.jp/crid/1360307817250276352; Nitrous Oxide (N ₂ O): The Dominant Ozone-Depleting Substance Emitted in the 21st Century; https://cir.nii.ac.jp/crid/1360574092886488832; Compilation and analysis of<i>Escherichia coli</i>promoter DNA sequences; https://cir.nii.ac.jp/crid/1360574096552615552; Genomics and Ecology of Novel N2O-Reducing Microorganisms; https://cir.nii.ac.jp/crid/1360855569299883520; Competition for electrons favours <scp> N ₂ O </scp> reduction in denitrifying <i>Bradyrhizobium</i> isolates; https://cir.nii.ac.jp/crid/1360857597946470400; FixK2 Is the Main Transcriptional Activator of Bradyrhizobium diazoefficiens nosRZDYFLX Genes in Response to Low Oxygen; https://cir.nii.ac.jp/crid/1360865821284066432; Genetic design of soybean hosts and bradyrhizobial endosymbionts reduces N2O emissions from soybean rhizosphere; https://cir.nii.ac.jp/crid/1360870492936004224; Denitrification of nitrate to nitrogen gas by washed cells ofRhizobium japonicum and by bacteroids fromGlycine max; https://cir.nii.ac.jp/crid/1360870768605032960; Regulation of nitrous oxide reductase genes by NasT‐mediated transcription antitermination in <i>Bradyrhizobium diazoefficiens</i>; https://cir.nii.ac.jp/crid/1361137045710577280; Disparate Oxygen Responsiveness of Two Regulatory Cascades That Control Expression of Symbiotic Genes in <i>Bradyrhizobium japonicum</i>; https://cir.nii.ac.jp/crid/1362544419123125504; Transmissible Resistance to Penicillin G, Neomycin, and Chloramphenicol in <i>Rhizobium japonicum</i>; https://cir.nii.ac.jp/crid/1363388846109345536; A comprehensive quantification of global nitrous oxide sources and sinks; https://cir.nii.ac.jp/crid/1363670320351029120; Diversity of Bradyrhizobium in non-leguminous sorghum plants: B. ottawaense isolates unique in genes for N2O reductase and lack of the type VI secretion system; https://cir.nii.ac.jp/crid/1390002184862414976; Symbiotic Bradyrhizobium japonicum reduces N_2O surrounding the soybean root system via nitrous oxide reductase; https://cir.nii.ac.jp/crid/1570009751549437824
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org
• 書誌ID（NDLBibID）: 034654841; http://id.ndl.go.jp/bib/034654841