PCR-RFLP法によるアリモドキゾウムシの沖縄系統と小笠原系統の識別

• 資料種別: 記事
• タイトル: PCR-RFLP法によるアリモドキゾウムシの沖縄系統と小笠原系統の識別
• 著者・編者: 川村 清久; 大野 豪; 原口 大 他
• 著者標目: 川村 清久; 大野 豪; 原口 大
• 並列タイトル等: PCR-RFLP discrimination between Okinawa and Ogasawara stains of the sweet potato weevil, Cylas formicarius (fabricius)
• タイトル（掲載誌）: 日本応用動物昆虫学会誌
• 巻号年月日等（掲載誌）: 53(2) 2009.5
• 掲載巻: 53
• 掲載号: 2
• 掲載ページ: 60～63
• 掲載年月日（W3CDTF）: 2009-05
• ISSN（掲載誌）: 0021-4914
• ISSN-L（掲載誌）: 0021-4914
• 出版事項（掲載誌）: 東京 : 日本応用動物昆虫学会
• 出版地（国名コード）: JP
• 本文の言語コード: jpn
• 件名標目: Cylas formicarius; marking; PCR-RFLP; sterile insect technique
• NDLC: ZR4
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z18-41
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 10320105; http://id.ndl.go.jp/bib/10320105
• 整理区分コード: 632

• 要約等: To establish a simple method for discriminating Okinawa and Ogasawara strains of the sweet potato weevil, <i>Cylas formicarius</i>, about 560 bp of the internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA (rDNA) of <i>C. formicarius</i> was collected from several islands of Okinawa Prefecture and Ogasawara Islands and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The 345 weevils collected in Okinawa and Ogasawara were discriminated correctly by the RFLP patterns with the restriction enzyme <i>Rsa</i>I. The results suggest that <i>C. formicarius</i> originating from Ogasawara Islands is an effective genetic marking strain for an eradication project using the sterile insect technique implemented by the Okinawa prefectural government.
• DOI: 10.1303/jjaez.2009.60
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: To establish a simple method for discriminating Okinawa and Ogasawara strains of the sweet potato weevil, <i>Cylas formicarius</i>, about 560 bp of the internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA (rDNA) of <i>C. formicarius</i> was collected from several islands of Okinawa Prefecture and Ogasawara Islands and analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The 345 weevils collected in Okinawa and Ogasawara were discriminated correctly by the RFLP patterns with the restriction enzyme <i>Rsa</i>I. The results suggest that <i>C. formicarius</i> originating from Ogasawara Islands is an effective genetic marking strain for an eradication project using the sterile insect technique implemented by the Okinawa prefectural government.
• DOI: 10.1303/jjaez.2009.60; https://doi.org/10.1303/jjaez.2009.60
• 関連情報（URI）: https://agriknowledge.affrc.go.jp/RN/2030773078; http://id.ndl.go.jp/bib/10320105; https://ndlsearch.ndl.go.jp/books/R000000004-I10320105
• 参照: Evaluation of the Ribosomal ITS2 DNA Sequences in Separating Closely Related Populations of the Parasitoid <I>Ageniaspis</I> (Hymenoptera: Encyrtidae); https://cir.nii.ac.jp/crid/1362544419310570624; Ribosomal ITS1 Polymorphisms in <I>Ceratitis capitata</I> and <I>Ceratitis rosa</I> (Diptera: Tephritidae); https://cir.nii.ac.jp/crid/1362544420062972160; Genetic variation of sweet potato weevils, Cylas formicarius (Fabricius) (Coleoptera: Brentidae), in main infested areas in the world based upon the internal transcribed spacer-1 (ITS-1) region; https://cir.nii.ac.jp/crid/1390282681221178368
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 学術機関リポジトリデータベース; https://irdb.nii.ac.jp; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 10320105; http://id.ndl.go.jp/bib/10320105
• NII論文ID: 130000145752