Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1

• 資料種別: 記事
• タイトル: Enhancement of β-glucosidase activity on the cell-surface of sake yeast by disruption of SED1
• 著者・編者: Atsushi Kotaka; Hiroshi Sahara; Kouichi Kuroda 他
• 著者標目: Atsushi Kotaka; Hiroshi Sahara; Kouichi Kuroda
• タイトル（掲載誌）: Journal of bioscience and bioengineering
• 巻号年月日等（掲載誌）: 109(5) 2010.5
• 掲載巻: 109
• 掲載号: 5
• 掲載ページ: 442～446
• 掲載年月日（W3CDTF）: 2010-05
• ISSN（掲載誌）: 1389-1723
• 出版事項（掲載誌）: Suita : Society for Biotechnology
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• 件名標目: SED1; Cell-surface engineering; Gene disruption; Diploid sake yeast; β-glucosidase
• NDLC: ZP15
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z53-S65
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 10692714; http://id.ndl.go.jp/bib/10692714
• 整理区分コード: 632

• 要約等: We determined the genetic background that would result in a more optimal display of heterologously expressed beta-glucosidase (BGL) on the cell surface of yeast Saccharomyces cerevisiae. Amongst a collection of 28 strains carrying deletions in genes for glycosylphosphatidyl inositol (GPI)-anchored proteins, the Delta sed1 and Delta tos6 strains had significantly higher BGL-activity whilst maintaining wild type growth. Absence of Sed1p, which might facilitate incorporation of anchored BGL on the cell-surface, could also influence the activity of BGL on the cell surface with the heterologous gene being placed under the control of the SED1 promoter. For the evaluation of its industrial applicability we tested this system in heterologous and homogenous SED1-disruptants of sake yeast, a diploid S. cerevisiae strain, in which either the SED1 ORF or the complete gene including the promoter was deleted by use of the high-efficiency loss of heterozygosity method. Evaluation of disruptants displaying BGL showed that deletion of the SED1 ORF enhanced BGL activity on the cell surface, while additional deletion of the SED1 promoter increased further BGL activity on the cell surface. Compared to heterozygous disruption, homozygous disruption resulted generally in a higher BGL activity. Thus, homozygous deletion of both SED1 gene and promoter resulted in the most efficient display of BGL reaching a 1.6-fold increase of BGL-activity compared to wild type.
• DOI: 10.1016/j.jbiosc.2009.11.003; https://doi.org/10.1016/j.jbiosc.2009.11.003
• 関連情報（URI）: http://id.ndl.go.jp/bib/10692714; https://ndlsearch.ndl.go.jp/books/R000000004-I10692714; https://api.elsevier.com/content/article/PII:S1389172309010135?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:S1389172309010135?httpAccept=text/plain
• 参照: Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation; https://cir.nii.ac.jp/crid/1360846640214572672; 平成22年度における酒類の研究業績; https://cir.nii.ac.jp/crid/1390282681103607168; Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain; https://cir.nii.ac.jp/crid/1521136280798755968; Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation; https://cir.nii.ac.jp/crid/1521980703391167360; The construction and application of diploid sake yeast with a homozygous mutation in the FAS2 gene; https://cir.nii.ac.jp/crid/1523951029617366656; Mutation in the peroxin-coding gene PEX22 contributing to high malate production in Saccharomyces cerevisiae; https://cir.nii.ac.jp/crid/1524232504689319680
• 参照: Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity; https://cir.nii.ac.jp/crid/1360011145280128000; Direct ethanol production from barley β-glucan by sake yeast displaying Aspergillus oryzae β-glucosidase and endoglucanase; https://cir.nii.ac.jp/crid/1360283694137018112; Global Gene Expression Analysis of Yeast Cells during Sake Brewing; https://cir.nii.ac.jp/crid/1360574093856220928; <i>SED1</i> Gene Length and Sequence Polymorphisms in Feral Strains of <i>Saccharomyces cerevisiae</i>; https://cir.nii.ac.jp/crid/1360574094112187392; PCR‐mediated seamless gene deletion and marker recycling in <i>Saccharomyces cerevisiae</i>; https://cir.nii.ac.jp/crid/1360574095103783040; Yeast cell-surface display?applications of molecular display; https://cir.nii.ac.jp/crid/1361137043962342016; Transformation of intact yeast cells treated with alkali cations; https://cir.nii.ac.jp/crid/1361137044305699584; Sed1p Is a Major Cell Wall Protein of <i>Saccharomyces cerevisiae</i> in the Stationary Phase and Is Involved in Lytic Enzyme Resistance; https://cir.nii.ac.jp/crid/1361137045188541184; Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in<i>Saccharomyces cerevisiae</i>cultures induce expression of intracellular metal metabolism genes regulated by Aft1p; https://cir.nii.ac.jp/crid/1361137045960941312; [31] Tackling the protease problem in Saccharomyces cerevisiae; https://cir.nii.ac.jp/crid/1361418520975222016; A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance; https://cir.nii.ac.jp/crid/1361699995253596160; Differential regulation of cell wall biogenesis during growth and development in yeast; https://cir.nii.ac.jp/crid/1362825896166832896; Isoflavone aglycones production from isoflavone glycosides by display of β-glucosidase from Aspergillus oryzae on yeast cell surface; https://cir.nii.ac.jp/crid/1363670320156573824; Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate; https://cir.nii.ac.jp/crid/1520854805784279680; PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vector; https://cir.nii.ac.jp/crid/1570009750598385920
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles; 科学研究費助成事業データベース; https://kaken.nii.ac.jp/ja
• 書誌ID（NDLBibID）: 10692714; http://id.ndl.go.jp/bib/10692714
• NII論文ID: 110007618918