Expression of the β-D-Glucosidase 1 Gene in Bifidobacterium breve 203 during Acclimation to Cellobiose

• 資料種別: 記事
• タイトル: Expression of the β-D-Glucosidase 1 Gene in Bifidobacterium breve 203 during Acclimation to Cellobiose
• 著者・編者: Naoki Nunoura; Kohji Ohdan; Kenji Yamamoto 他
• 著者標目: Naoki Nunoura; Kohji Ohdan; Kenji Yamamoto
• タイトル（掲載誌）: Journal of Fermentation and Bioengineering
• 巻号年月日等（掲載誌）: 83(4) 1997.04
• 掲載巻: 83
• 掲載号: 4
• 掲載ページ: 309～314
• 掲載年月日（W3CDTF）: 1997-04
• ISSN（掲載誌）: 0922-338X
• 出版事項（掲載誌）: Suita : Society of Fermentation and Bioengineering
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• NDLC: ZP15
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z53-S65
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 4209651; http://id.ndl.go.jp/bib/4209651
• 整理区分コード: 632

• 要約等: Abstract The β- d -glucosidase I (EC 3.2.1.21) activity of Bifidobacterium breve 203 isolated from feces of adult humans was found to be very low, but when B. breve 203 was acclimated to cellobiose, the activity of the enzyme increased in the acclimated cell, B. breve 203 clb. With the aim of elucidating the mechanism, responsible for this increased activity the translation and transcription of the β- d -glucosidase I gene were investigated using an antibody against β- d -glucosidase I and a probe specific to the gene, respectively. Western and Northern blots showed marked differences in the expression of the enzyme and the level of its mRNA between B. breve 203 and B. breve 203 clb. However, genomic Southern blots revealed only insignificant differences in the copy numbers of the open reading frames in the two strains. The 5′ flanking regions of the β- d -glucosidase I gene of B. breve 203 and that of B. breve 203 clb were therefore analyzed. The transcriptional start point, located 94 bp upstream from the first methionine codon, was identified by primer extension. A sequence, -TTGGAA-(15 bp)-TAATCT-, located 8 bp upstream from the transcriptional start point, was assigned as the promoter of the gene. A sequence highly homologous to the lac operator region of Escherichia coli was found downstream of the transcriptional start point.
• DOI: 10.1016/s0922-338x(97)80134-1; https://doi.org/10.1016/s0922-338x(97)80134-1
• 関連情報（URI）: http://id.ndl.go.jp/bib/4209651; https://ndlsearch.ndl.go.jp/books/R000000004-I4209651; https://api.elsevier.com/content/article/PII:S0922338X97801341?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:S0922338X97801341?httpAccept=text/plain
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• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 4209651; http://id.ndl.go.jp/bib/4209651
• NII論文ID: 110002682500