Dental pulp cells with multi-potential for differentiation to odontoblast and chondroblast

• 資料種別: 記事
• タイトル: Dental pulp cells with multi-potential for differentiation to odontoblast and chondroblast
• 著者・編者: Kikuji Yamashita; James E. Dennis; Donald P. Lennon 他
• 著者標目: Kikuji Yamashita; James E. Dennis; Donald P. Lennon
• タイトル（掲載誌）: Journal of hard tissue biology
• 巻号年月日等（掲載誌）: 12(2) 2003.12
• 掲載巻: 12
• 掲載号: 2
• 掲載ページ: 49～55
• 掲載年月日（W3CDTF）: 2003-12
• ISSN（掲載誌）: 1341-7649
• ISSN-L（掲載誌）: 1341-7649
• 出版事項（掲載誌）: [Okayama] : The Hard Tissue Biology Network Association
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• 件名標目: dental pulp cell; odontoblast; chondroblast; multi-potential; pellet culture
• NDLC: ZS7
• 対象利用者: 一般
• 所蔵機関: 国立国会図書館
• 請求記号: Z19-B312
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 6960919; http://id.ndl.go.jp/bib/6960919
• 整理区分コード: 632

• 要約等: The repair of dentin is thought to be maintained through differentiation of undefined precursor cells within the dental pulp to odontoblasts. To investigate the characteristics of these undefined precursor cells in dental pulp, mixed pulp cells and cloned cells were obtained from <i>H-2K^b-ts A58</i> heterozygous transgenic mice. Differentiation of dental pulp precursor cells <i>in vitro</i> was assayed by RT-PCR analysis of gene expression of osteopontin, osteocalcin and dentin sialophosphoprotein (DSPP), and by von Kossa staining for mineralization. All assays were conducted on cultured mixed pulp cells and clonal cells PC1, PC8 and PC14. The results suggested that dental pulp cells could differentiate to odontoblasts <i>in vitro</i>. To test for chondrogenic potential, mixed pulp cells and clonal cells PC1, PC8 and PC14 were maintained in high density pellet cultures, and PC1 cells were stained with toluidine blue for glycosaminoglycans (GAG) content. To confirm the chondrocyte phenotype, the extracellular matrix of PC1 cells in pellet culture was stained with anti-type II collagen antibody. This study is the first to demonstrate that dental pulp stem cells present in the dental pulp have multi-potential to differentiate to both odontoblast and chondroblast.
• DOI: 10.2485/jhtb.12.49
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: The repair of dentin is thought to be maintained through differentiation of undefined precursor cells within the dental pulp to odontoblasts. To investigate the characteristics of these undefined precursor cells in dental pulp, mixed pulp cells and cloned cells were obtained from <i>H-2K^b-ts A58</i> heterozygous transgenic mice. Differentiation of dental pulp precursor cells <i>in vitro</i> was assayed by RT-PCR analysis of gene expression of osteopontin, osteocalcin and dentin sialophosphoprotein (DSPP), and by von Kossa staining for mineralization. All assays were conducted on cultured mixed pulp cells and clonal cells PC1, PC8 and PC14. The results suggested that dental pulp cells could differentiate to odontoblasts <i>in vitro</i>. To test for chondrogenic potential, mixed pulp cells and clonal cells PC1, PC8 and PC14 were maintained in high density pellet cultures, and PC1 cells were stained with toluidine blue for glycosaminoglycans (GAG) content. To confirm the chondrocyte phenotype, the extracellular matrix of PC1 cells in pellet culture was stained with anti-type II collagen antibody. This study is the first to demonstrate that dental pulp stem cells present in the dental pulp have multi-potential to differentiate to both odontoblast and chondroblast.
• DOI: 10.2485/jhtb.12.49; https://doi.org/10.2485/jhtb.12.49
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（URI）: http://id.ndl.go.jp/bib/6960919; https://ndlsearch.ndl.go.jp/books/R000000004-I6960919; https://search.jamas.or.jp/link/ui/2006013603
• 参照: Alterations in Deciduous Dental Pulp Cells Cultured with Serum-free Medium; https://cir.nii.ac.jp/crid/1390282679435923456
• 参照: Odontoblast commitment and differentiation; https://cir.nii.ac.jp/crid/1360011143796651008; Direct derivation of conditionally immortal cell lines from an H-2Kb-tsA58 transgenic mouse.; https://cir.nii.ac.jp/crid/1360011145419114496; Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures; https://cir.nii.ac.jp/crid/1360011145776957952; Muscle Regeneration by Bone Marrow-Derived Myogenic Progenitors; https://cir.nii.ac.jp/crid/1360855570528762368; Single-Colony Derived Strains of Human Marrow Stromal Fibroblasts Form Bone After Transplantation In Vivo; https://cir.nii.ac.jp/crid/1361418519007061888; Expression of Osteopontin in the Inner Ear^a; https://cir.nii.ac.jp/crid/1361699993555454464; Postnatal human dental pulp stem cells (DPSCs) <i>in vitro</i> and <i>in</i> <i>vivo</i>; https://cir.nii.ac.jp/crid/1361981470846498048; Morphogenesis of mineralized tissues induced by neonatal mouse molar pulp isografts in the spleen; https://cir.nii.ac.jp/crid/1362262946435714944; Differentiation of Pulpal Cells and Inductive Influences of Various Matrices with Reference to Pulpal Wound Healing; https://cir.nii.ac.jp/crid/1362544421395810816; Osteopontin and the Bone Remodeling Sequence; https://cir.nii.ac.jp/crid/1363388844782354432; Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats—similarities to astrocyte grafts; https://cir.nii.ac.jp/crid/1363951793558911872; Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone; https://cir.nii.ac.jp/crid/1364233269333603200
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 6960919; http://id.ndl.go.jp/bib/6960919
• NII論文ID: 130004480419; 110001142070