Laboratory investigation: Expression and distribution of junctional adhesion molecule-1 in the human cornea

• 資料種別: 記事
• タイトル: Laboratory investigation: Expression and distribution of junctional adhesion molecule-1 in the human cornea
• 著者・編者: Lizhong Chen; Nobuyuki Ebihara; Keiko Fujiki 他
• 著者標目: Lizhong Chen; Nobuyuki Ebihara; Keiko Fujiki
• タイトル（掲載誌）: Japanese journal of ophthalmology : the official international journal of the Japanese Ophthalmological Society / Japanese Ophthalmological Society
• 巻号年月日等（掲載誌）: 51(6) 2007.11・12
• 掲載巻: 51
• 掲載号: 6
• 掲載ページ: 405～411
• 掲載年月日（W3CDTF）: 2007
• ISSN（掲載誌）: 0021-5155
• 出版事項（掲載誌）: [Tokyo] : [Springer Japan]
• 出版地（国名コード）: JP
• 本文の言語コード: eng
• 件名標目: junction adhesion molecule-1; keratocyte; myofibroblast; occludin; tight junction
• NDLC: ZS42
• 対象利用者: 一般
• レポート番号（雑誌記事）: DOI10.1007/s10384-007-0479-5
• 所蔵機関: 国立国会図書館
• 請求記号: Z53-D450
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 9302003; http://id.ndl.go.jp/bib/9302003
• 整理区分コード: 632

• 要約等: To investigate the expression and distribution of junctional adhesion molecule 1 (JAM-1) in human corneal tissue and cells.Reverse transcriptase-polymerase chain reaction was used to detect the expression of JAM-1, ZO-1, and occludin mRNAs in corneal cells, while the presence of JAM-1 protein was analyzed by flow cytometry (FACS). Double immunofluorescence staining was used to determine the tissue distribution of JAM-1 and occludin in human corneas.Strong expression of JAM-1, ZO-1, and occludin mRNAs was observed in primary cultured corneal epithelial and endothelial cells, but not in primary cultured keratocytes. The expression of JAM-1 protein in cultured epithelial and endothelial cells was confirmed by FACS. When keratocytes were cultured in medium with 10% fetal calf serum for several passages, differentiation into corneal myofibroblasts occurred. The expression of JAM-1 was detected in these corneal myofibroblasts at both RNA and protein levels. JAM-1 immunoreactivity was seen at cell borders throughout the entire epithelium, but not in keratocytes from normal corneal tissue. On the other hand, JAM-1 immunoreactivity was detected in the cytoplasm of corneal endothelial cells.JAM-1 is expressed by human corneal epithelial and endothelial cells, but not by keratocytes, although its expression is induced in corneal myofibroblasts.
• DOI: 10.1007/s10384-007-0479-5; https://doi.org/10.1007/s10384-007-0479-5
• 関連情報（URI）: http://id.ndl.go.jp/bib/9302003; https://ndlsearch.ndl.go.jp/books/R000000004-I9302003; http://link.springer.com/content/pdf/10.1007/s10384-007-0479-5.pdf; http://link.springer.com/article/10.1007/s10384-007-0479-5/fulltext.html; http://link.springer.com/content/pdf/10.1007/s10384-007-0479-5; https://search.jamas.or.jp/link/ui/2008172600
• 参照: Involvement of Nectin–Afadin in the Adherens Junctions of the Corneal Endothelium; https://cir.nii.ac.jp/crid/1360004234891917440
• 参照: The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM); https://cir.nii.ac.jp/crid/1360011145339521920; Junctional Adhesion Molecule Interacts with the PDZ Domain-containing Proteins AF-6 and ZO-1; https://cir.nii.ac.jp/crid/1360292619854024704; Tight junction-related protein expression and distribution in human corneal epithelium; https://cir.nii.ac.jp/crid/1360292620136936960; Keratocyte and fibroblast phenotypes in the repairing cornea; https://cir.nii.ac.jp/crid/1360292620428516096; Corneal Keratocytes: Phenotypic and Species Differences in Abundant Protein Expression and In Vitro Light-Scattering; https://cir.nii.ac.jp/crid/1360292620738148224; CD-34 stromal expression pattern in normal and altered human corneas; https://cir.nii.ac.jp/crid/1360574096038810624; Interaction of Junctional Adhesion Molecule with the Tight Junction Components ZO-1, Cingulin, and Occludin; https://cir.nii.ac.jp/crid/1360855568535319936; Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction; https://cir.nii.ac.jp/crid/1360855571357619072; Involvement of the Junctional Adhesion Molecule-1 (JAM1) Homodimer Interface in Regulation of Epithelial Barrier Function; https://cir.nii.ac.jp/crid/1361137043665952896; Junctional Adhesion Molecule 1 Regulates Epithelial Cell Morphology through Effects on β1 Integrins and Rap1 Activity; https://cir.nii.ac.jp/crid/1361137045839963776; ULTRASTRUCTURE AND DISTRIBUTION OF INTERCELLULAR JUNCTIONS IN CORNEAL ENDOTHELIUM; https://cir.nii.ac.jp/crid/1361418518632867072; Antibody Blockade of Junctional Adhesion Molecule-A in Rabbit Corneal Endothelial Tight Junctions Produces Corneal Swelling; https://cir.nii.ac.jp/crid/1361418518831133952; The JAM family of proteins; https://cir.nii.ac.jp/crid/1361418520455694720; Junctional Adhesion Molecules (JAMs) are Differentially Expressed in Fibroblasts and Co-Localize with ZO-1 to Adherens-Like Junctions; https://cir.nii.ac.jp/crid/1361418521192559488; Identification and characterisation of human Junctional Adhesion Molecule (JAM); https://cir.nii.ac.jp/crid/1361699995787091584; Changes in Corneal Endothelial Apical Junctional Protein Organization After Corneal Cold Storage; https://cir.nii.ac.jp/crid/1361981469557140480; Keratocyte Phenotype Mediates Proteoglycan Structure; https://cir.nii.ac.jp/crid/1362262944638274816; ZO1 in Corneal Epithelium: Association to the Zonula Occludens and Adherens Junctions; https://cir.nii.ac.jp/crid/1362262945832963840; Deletion of JAM-A causes morphological defects in the corneal epithelium; https://cir.nii.ac.jp/crid/1362262946066837760; Expression of CD34 and L-Selectin on Human Corneal Keratocytes; https://cir.nii.ac.jp/crid/1362544418291849344; Junctional adhesion molecule (JAM) binds to PAR-3; https://cir.nii.ac.jp/crid/1362544421336794624; Multi-PDZ Domain Protein 1 (MUPP1) Is Concentrated at Tight Junctions through Its Possible Interaction with Claudin-1 and Junctional Adhesion Molecule; https://cir.nii.ac.jp/crid/1362825895627581824; Junctional Adhesion Molecule, a Novel Member of the Immunoglobulin Superfamily That Distributes at Intercellular Junctions and Modulates Monocyte Transmigration; https://cir.nii.ac.jp/crid/1363107370947408000; Association of Junctional Adhesion Molecule with Calcium/calmodulin-dependent Serine Protein Kinase (CASK/LIN-2) in Human Epithelial Caco-2 Cells; https://cir.nii.ac.jp/crid/1363388843744691584; Induction of ??-Smooth Muscle Actin Expression and Myofibroblast Transformation in Cultured Corneal Keratocytes; https://cir.nii.ac.jp/crid/1363670319020031232; Characterization of huJAM: evidence for involvement in cell-cell contact and tight junction regulation; https://cir.nii.ac.jp/crid/1363951793756901248; Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins; https://cir.nii.ac.jp/crid/1364233270956564608; The spatial organization of apical junctional complex-associated proteins in feline and human corneal endothelium; https://cir.nii.ac.jp/crid/1364233271187631744
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles
• 書誌ID（NDLBibID）: 9302003; http://id.ndl.go.jp/bib/9302003
• NII論文ID: 10020178893