TAT-mediated peroxiredoxin 5 and 6 protein transduction protect against high glucose-induced cytotoxicity in retinal pericytes

• 資料種別: 文書・図像類
• タイトル: TAT-mediated peroxiredoxin 5 and 6 protein transduction protect against high glucose-induced cytotoxicity in retinal pericytes
• 著者・編者: KUBO, Eri; Singh, Dhirendra. P.; Fatma, Nigar; AKAGI, Yoshio
• 著者標目: KUBO, Eri; Singh, Dhirendra. P.; Fatma, Nigar; AKAGI, Yoshio
• 出版事項: ELSEVIER SCIENCE INC
• 出版年月日等: 2009-06
• 出版年（W3CDTF）: 2009-06
• タイトル（掲載誌）: Life Science
• 巻号年月日等（掲載誌）: 84
• 掲載巻: 84
• 掲載ページ: 857-864
• 本文の言語コード: eng
• 件名標目: Retinopathy; Pericyte; Oxidative stress; Diabetes; Peroxiredoxin
• 対象利用者: 一般
• 一般注記: Aims: Hyperglycemia-induced oxidative stress is implicated to be involved in pericyte apoptosis seen in diabetic retinopathy. The six mammalian Peroxiredoxins (PRDXs) comprise a novel family of antioxidative proteins that negatively regulate oxidative stress-induced apoptosis by controlling reactive oxygen species (ROS) levels. Main methods: Sprague Dawley rats were used to detect the retinal expressions of PRDXs1-6. Pig pericytes cultured in high-glucose medium were used to monitor the protective effect of PRDX5 and 6 against high-glucose- associated change. Recombinant PRDX5 and 6 proteins were linked to the Trans-Activating Transduction (TAT) domain from HIV-1 TAT protein for their efficient delivery into cells/tissues. Key findings: We found higher expression of PRDX5 and 6 mRNAs and PRDX5 and 6 proteins in retina than the other Prdxs (Prdx 1–4). Western blotting affirmed the intracellular presence of TAT-linked proteins and revealed the efficient transduction of TAT-HA-PRDX5 and 6 in these cells. Extrinsic supply of TAT-HA-PRDX5 and 6 proteins inhibited the oxidative stress induced DNA damage after high-glucose exposure in pig pericytes. The cell survival and apoptosis assay revealed that extrinsic supply of TATHA- PRDX5 and 6 proteins were responsible for inhibiting hyperglycemia-induced pericyte 2 apoptosis. Significance: Results suggest that delivery of PRDX5 and 6 might protect hyperglycemiainduced pericyte loss to inhibit oxidative stress.
• 一次資料へのリンクURL: https://u-fukui.repo.nii.ac.jp/?action=repository_action_common_download&item_id=19826&item_no=1&attribute_id=22&file_no=1
• オンライン閲覧公開範囲: 限定公開
• 関連情報（DOI）: 10.1016/j.lfs.2009.03.019
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 福井大学 : 福井大学学術機関リポジトリ