Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts

• 資料種別: 博士論文
• タイトル: Biological impacts of resveratrol, quercetin, and N-acetylcysteine on oxidative stress in human gingival fibroblasts
• 著者・編者: Orihuela Campos, Rita Cristina; Tamaki, Naofumi; Mukai, Rie; Fukui, Makoto; Miki, Kaname; Terao, Junji; Ito, Hiro-O
• 著者標目: Orihuela Campos, Rita Cristina; Tamaki, Naofumi; Mukai, Rie; Fukui, Makoto; Miki, Kaname; Terao, Junji; Ito, Hiro-O
• 出版事項: SOCIETY FOR FREE RADICAL RESEARCH JAPAN
• 出版年月日等: 2015-05-01
• 出版年（W3CDTF）: 2015-05-01
• 並列タイトル等: ヒト歯肉線維芽細胞における酸化ストレスに対するレスベラトロール，ケルセチン，及びN-アセチルシステインの生物学的影響
• タイトル（掲載誌）: Journal of Clinical Biochemistry and Nutrition
• 巻号年月日等（掲載誌）: 56 3
• 掲載巻: 56
• 掲載号: 3
• 掲載ページ: 220-227
• ISSN（掲載誌）: ISSN : 1880-5086; ISSN : 0912-0009
• 授与機関名: 徳島大学
• 授与年月日: 2015-03-23
• 報告番号: 甲第2810号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: human gingival fibroblasts; periodontitis; resveratrol; quercetin; N-acetylcysteine
• 対象利用者: 一般
• 一般注記: In periodontitis, production of reactive oxygen species (ROS) by neutrophils induces oxidative stress and deteriorates surrounding tissues. Antioxidants reduce damage caused by ROS and are used to treat diseases involving oxidative stress. This study summarizes the different effects of resveratrol, quercetin, and N-acetylcysteine (NAC) on human gingival fibroblasts (HGFs) under oxidative stress induced by hydrogen peroxide. Real-time cytotoxicity analyses reveals that resveratrol and quercetin enhanced cell proliferation even under oxidative stress. Of the antioxidants tested, resveratrol is the most effective at inhibiting ROS production. HGFs incubated with resveratrol and quercetin up-regulate the transcription of type I collagen gene after 3 h, but only resveratrol sustained this up-regulation for 24 h. A measurement of the oxygen consumption rate (OCR, mitochondrial respiration) shows that resveratrol generates the highest maximal respiratory capacity, followed by quercetin and NAC. Simultaneous measurement of OCR and the extracellular acidification rate (non-mitochondrial respiration) reveals that resveratrol and quercetin induce an increase in mitochondrial respiration when compared with untreated cells. NAC treatment consumes less oxygen and enhances more non-mitochondrial respiration. In conclusion, resveratrol is the most effective antioxidant in terms of real-time cytotoxicity analysis, reduction of ROS production, and enhancement of type I collagen synthesis and mitochondrial respiration in HGFs.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: Copyright © 2015 日本酸化ストレス学会(Society for Free Radical Research Japan)
• 関連情報（DOI）: 10.3164/jcbn.14-129
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/10/109399/20170929143512134096/LID201505291001.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109399/20170929143513303588/LID201505291002.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109399/20170929143513645149/LID201507171007.pdf fulltext