Double Stranded RNA-Dependent Protein Kinase is Necessary for TNF-α-Induced Osteoclast Formation In Vitro and In Vivo

• 資料種別: 博士論文
• タイトル: Double Stranded RNA-Dependent Protein Kinase is Necessary for TNF-α-Induced Osteoclast Formation In Vitro and In Vivo
• 著者・編者: Shinohara, Hiroki; Teramachi, Jumpei; Okamura, Hirohiko; Yang, Di; Nagata, Toshihiko; Haneji, Tatsuji
• 著者標目: Shinohara, Hiroki; Teramachi, Jumpei; Okamura, Hirohiko; Yang, Di; Nagata, Toshihiko; Haneji, Tatsuji
• 出版事項: Wiley
• 出版年月日等: 2015-03-04
• 出版年（W3CDTF）: 2015-03-04
• 並列タイトル等: 二本鎖RNA依存性プロテインキナーゼは in vitroとin vivo においてTNF-αによる破骨細胞形成に必要である; PKR is necessary for osteoclastogenesis.
• タイトル（掲載誌）: Journal of Cellular Biochemistry
• 巻号年月日等（掲載誌）: 116 9
• 掲載巻: 116
• 掲載号: 9
• 掲載ページ: 1957-1967
• ISSN（掲載誌）: ISSN : 0730-2312; ISSN : 1097-4644
• 授与機関名: 徳島大学
• 授与年月日: 2015-06-11
• 報告番号: 甲第2857号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: PKR; OSTEOCLAST; RAW264.7; TNF-α; NF-κB; MAPK
• 対象利用者: 一般
• 一般注記: Double-stranded RNA-dependent protein kinase (PKR) is involved in cell cycle progression, cell proliferation, cell differentiation, tumorgenesis, and apoptosis. We previously reported that PKR is required for differentiation and calcification in osteoblasts. TNF-α plays a key role in osteoclast differentiation. However, it is unknown about the roles of PKR in the TNF-α-induced osteoclast differentiation. The expression of PKR in osteoclast precursor RAW264.7 cells increased during TNF-α-induced osteoclastogenesis. The TNF-α-induced osteoclast differentiation in bone marrow-derived macrophages and RAW264.7 cells was markedly suppressed by the pre-treatment of PKR inhibitor, 2-aminopurine (2AP), as well as gene silencing of PKR. The expression of gene markers in the differentiated osteoclasts including TRAP, Calcitonin receptor, cathepsin K and ATP6V0d2 was also suppressed by the 2AP treatment. Bone resorption activity of TNF-α-induced osteoclasts was also supressed by 2AP treatment. Inhibition of PKR supressed the TNF-α-induced activation of NF-κB and MAPK in RAW264.7 cells. 2AP inhibited both the nuclear translocation of NF-κB and its transcriptional activity in RAW264.7 cells. 2AP inhibited the TNF-α-induced expression of NFATc1 and c-fos, master transcription factors in osteoclastogenesis. TNF-α-induced nuclear translocation of NFATc1 in mature osteoclasts was clearly inhibited by the 2AP treatment. The PKR inhibitor C16 decreased the TNF-α-induced osteoclast formation and bone resorption in mouse calvaria. The present study indicates that PKR is necessary for the TNF-α-induced osteoclast differentiation in vitro and in vivo.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is the peer reviewed version of the following article: Shinohara, H. , Teramachi, J. , Okamura, H. , Yang, D. , Nagata, T. and Haneji, T. (2015), Double Stranded RNA‐Dependent Protein Kinase is Necessary for TNF‐α‐Induced Osteoclast Formation In Vitro and In Vivo. J. Cell. Biochem., 116: 1957-1967, which has been published in final form at https://doi:10.1002/jcb.25151. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
• 関連情報（DOI）: 10.1002/jcb.25151
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/10/109478/20170929143942246509/LID201507171001.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109478/20170929143942914914/LID201507171002.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109478/20190927145607965056/k2857_fulltext.pdf fulltext