Sp6 regulation of Rock1 promoter activity in dental epithelial cells

• 資料種別: 博士論文
• タイトル: Sp6 regulation of Rock1 promoter activity in dental epithelial cells
• 著者・編者: Yanuarysuka, Ryna Dwi; Miyoshi, Keiko; Adiningrat, Arya; Horiguchi, Taigo; Tanimura, Ayako; Hagita, Hiroko; Noma, Takafumi
• 著者標目: Yanuarysuka, Ryna Dwi; Miyoshi, Keiko; Adiningrat, Arya; Horiguchi, Taigo; Tanimura, Ayako; Hagita, Hiroko; Noma, Takafumi
• 出版年月日等: 2014-08
• 出版年（W3CDTF）: 2014-08
• 並列タイトル等: 歯原性上皮細胞におけるSp6タンパク質によるRock1遺伝子プロモーター活性の制御; Sp6 regulates Rock1
• タイトル（掲載誌）: The journal of medical investigation : JMI
• 巻号年月日等（掲載誌）: 61 3-4
• 掲載巻: 61
• 掲載号: 3-4
• 掲載ページ: 306-317
• ISSN（掲載誌）: ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2014-09-10
• 報告番号: 甲第2756号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: amelogenesis; dental epithelial cells; Rock1; Sp6; transcription start site
• 対象利用者: 一般
• 一般注記: Sp6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. However, the underlying molecular mechanisms remain unclear. We have previously identified one of the Sp6 downstream genes, Rock1, which is involved in ameloblast polarization. In this study, we investigated the transcriptional regulatory mechanisms of Rock1 by Sp6. First, we identified the transcription start sites (TSS) and cloned the 5'- flanking region of Rock1. Serial deletion analyses identified a critical region for Rock1 promoter activity within the 249-bp upstream region of TSS, and chromatin immunoprecipitation assays revealed Sp6-binding to this region. Subsequent transient transfection experiments showed that Rock1 promoter activity is enhanced by Sp6, but reduced by Sp1. Treatment of dental epithelial cells with the GC-selective DNA binding inhibitor, mithramycin A, affected Rock1 promoter activity in loss of enhancement by Sp6, but not repression by Sp1. Further site-directed mutagenesis indicated that the region from -206 to -150 contains responsive elements for Sp6. Taken together, we conclude that Sp6 positively regulates Rock1 transcription by direct binding to the Rock1 promoter region from -206 to -150, which functionally distinct from Sp1.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.61.306
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/10/109578/20190724164259960061/LID201409191004.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109578/20190724164300578177/LID201409191005.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/10/109578/20190730171001564403/LID201503101001.pdf fulltext