Proteomic Analysis of Human Tendon and Ligament : Solubilization and Analysis of Insoluble Extracellular Matrix in Connective Tissues

• 資料種別: 博士論文
• タイトル: Proteomic Analysis of Human Tendon and Ligament : Solubilization and Analysis of Insoluble Extracellular Matrix in Connective Tissues
• 著者・編者: Sato, Nori; Taniguchi, Takako; Goda, Yuichiro; Kosaka, Hirofumi; Higashino, Kosaku; Sakai, Toshinori; Katoh, Shinsuke; Yasui, Natsuo; Sairyo, Koichi; Taniguchi, Hisaaki
• 著者標目: Sato, Nori; Taniguchi, Takako; Goda, Yuichiro; Kosaka, Hirofumi; Higashino, Kosaku; Sakai, Toshinori; Katoh, Shinsuke; Yasui, Natsuo; Sairyo, Koichi; Taniguchi, Hisaaki
• 出版事項: ACS Publications
• 出版年月日等: 2016-10-17
• 出版年（W3CDTF）: 2016-10-17
• 並列タイトル等: ヒト腱・靭帯のプロテオーム解析 : 結合組織における不溶性細胞外マトリックスの可溶化と解析
• タイトル（掲載誌）: Journal of Proteome Research
• 巻号年月日等（掲載誌）: 15 12
• 掲載巻: 15
• 掲載号: 12
• 掲載ページ: 4709-4721
• ISSN（掲載誌）: ISSN : 1535-3893
• 授与機関名: 徳島大学
• 授与年月日: 2017-02-23
• 報告番号: 甲第3009号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: 不溶性細胞外マトリックス; コラーゲン; エラスチン; プロテオーム解析; yellow ligament; Achilles tendon; elastin; collagen; proteome
• 対象利用者: 一般
• 一般注記: Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: Copyright © 2016 American Chemical Society
• 関連情報（DOI）: 10.1021/acs.jproteome.6b00806
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/110137/20170929142449609034/LID201705191001.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/110137/20180613155943282942/k3009_fulltext.pdf fulltext