Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria

• 資料種別: 博士論文
• タイトル: Antibacterial and Antibiofilm Effects of Reuterin-Related Compounds to Periodontopathic Bacteria
• 著者・編者: Fujiwara, Natsumi; Murakami, Keiji; Nakao, Michiyasu; Toguchi, Munehisa; Yumoto, Hiromichi; Hirota, Katsuhiko; Matsuo, Takashi; Sano, Shigeki; Ozaki, Kazumi; Miyake, Yoichiro
• 著者標目: Fujiwara, Natsumi; Murakami, Keiji; Nakao, Michiyasu; Toguchi, Munehisa; Yumoto, Hiromichi; Hirota, Katsuhiko; Matsuo, Takashi; Sano, Shigeki; Ozaki, Kazumi; Miyake, Yoichiro
• 出版事項: 四国歯学会
• 出版年月日等: 2017-06-30
• 出版年（W3CDTF）: 2017-06-30
• 並列タイトル等: ロイテリン関連化合物の歯周病原因菌への抗菌および抗バイオフィルム効果
• タイトル（掲載誌）: Journal of Oral Health and Biosciences
• 巻号年月日等（掲載誌）: 30 1
• 掲載巻: 30
• 掲載号: 1
• 掲載ページ: 8-17
• ISSN（掲載誌）: ISSN : 2189-6682
• 授与機関名: 徳島大学
• 授与年月日: 2017-03-09
• 報告番号: 甲第3076号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Lactobacillus reuteri; reuterin; Fusobacterium nucleatum; biofilm
• 対象利用者: 一般
• 一般注記: OBJECTIVE: Lactobacillus reuteri is one of the probiotics that possesses preventive effects on oral infections including dental caries and periodontal disease. Reuterin is a bacteriocin-like compound produced by L. reuteri and plays an important role in the probiotic effects. However, it is difficult to obtain reuterin due to the general lability of its aldehyde moiety. Therefore, fourteen stable reuterin-related compounds (RRCs) were chemically synthesized. Here, we investigated the effects of RRCs on periodontopathic bacteria. MATERIALS AND METHODS: The antibacterial activity of RRCs on pathogenic microorganisms including periodontopathic bacteria was determined. Moreover, the effects of RRCs on biofilm formation by Fusobacterium nucleatum were examined by a crystal violet biofilm formation assay. Cytotoxicity of RRCs was evaluated by a lactate dehydrogenase assay using oral keratinocytes. RESULTS: Among synthesized RRCs, RRC-04, -05, -09, -10, -12, -13, and -14, exhibited antibacterial activities against periodontopathic bacteria. RRCs, except for RRC-06, -07, -08, and -11, significantly suppressed biofilm formation to 60-80 % of the control. Most RRCs, except for RRC-12 and -13, were not cytotoxic to human oral keratinocytes. CONCLUSIONS: The synthesized RRCs can be potent novel oral care products for the prevention of periodontitis without adverse effects.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.20738/johb.30.1_8
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

https://repo.lib.tokushima-u.ac.jp/files/public/11/111063/20190820162137869064/LID201704261002.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111063/20190820162138194738/LID201704261003.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111063/20190820162138498710/k3076_fulltext.pdf fulltext