A Pseudomonas aeruginosa Quorum-Sensing autoinducer analog enhances the activity of antibiotics against resistant strains

• 資料種別: 博士論文
• タイトル: A Pseudomonas aeruginosa Quorum-Sensing autoinducer analog enhances the activity of antibiotics against resistant strains
• 著者・編者: Amoh, Takashi; Murakami, Keiji; Kariyama, Reiko; Hori, Kenji; Irie, Yasuhiko; Viducic, Darija; Hirota, Katsuhiko; Igarashi, Jun; Suga, Hiroaki; Kumon, Hiromi; Miyake, Yoichiro
• 著者標目: Amoh, Takashi; Murakami, Keiji; Kariyama, Reiko; Hori, Kenji; Irie, Yasuhiko; Viducic, Darija; Hirota, Katsuhiko; Igarashi, Jun; Suga, Hiroaki; Kumon, Hiromi; Miyake, Yoichiro
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2017-02
• 出版年（W3CDTF）: 2017-02
• 並列タイトル等: 緑膿菌クオラムセンシングオートインデューサー類似化合物は耐性菌に対し抗菌薬の活性を増強する; Enhancement activity of QS autoinducer analog
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 64 1-2
• 掲載巻: 64
• 掲載号: 1-2
• 掲載ページ: 101-109
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2017-03-23
• 報告番号: 甲第3078号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Pseudomonas aeruginosa; CRE; antibiotic resistance
• 対象利用者: 一般
• 一般注記: In this study, we have investigated the effects of the newly synthesized analog of Pseudomonas aeruginosa quorum-sensing autoinducer named AIA-1 (autoinducer analog) against antibiotic-resistant bacteria. In vitro susceptibility and killing assays for P. aeruginosa PAO1ΔoprD mutant and clinical isolates were performed by using antibiotics and AIA-1. In an in vivo assay, a luminescent carbapenem-resistant strain derived from PAO1ΔoprD was injected into neutropenic ICR mice and bioluminescence images were acquired after the treatment with antibiotics and AIA-1. Additionally, we investigated the effects of the combination use against carbapenem- resistant Enterobacteriaceae (CRE). Using killing assays in P. aeruginosa, the survival rates in the presence of antibiotics and AIA-1 significantly decreased in comparison with those with antibiotics alone. Furthermore, dual treatment of biapenem and AIA-1 was more effective than biapenem alone in a mouse infection model. AIA-1 did not change the MICs in P. aeruginosa, suggesting that AIA-1 acts on the mechanism of antibiotic tolerance. Conversely, the MICs of antibiotics decreased in the presence of AIA-1 in some CRE strains, indicating that AIA-1 may require additional mechanism to act on CRE. In conclusion, AIA-1 may be a potent drug for clinical treatment of infections caused by antibiotic-resistant bacteria.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.64.101
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

https://repo.lib.tokushima-u.ac.jp/files/public/11/111094/20190723113903402645/LID201704261006.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111094/20190723113903733145/LID201704261007.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111094/20190730170713788192/LID201704261008.pdf fulltext