Lung regeneration by fetal lung tissue implantation in a mouse pulmonary emphysema model

• 資料種別: 博士論文
• タイトル: Lung regeneration by fetal lung tissue implantation in a mouse pulmonary emphysema model
• 著者・編者: Uyama, Koh; Sakiyama, Shoji; Yoshida, Mitsuteru; Kenzaki, Koichiro; Toba, Hiroaki; Kawakami, Yukikiyo; Okumura, Kazumasa; Takizawa, Hiromitsu; Kondo, Kazuya; Tangoku, Akira
• 著者標目: Uyama, Koh; Sakiyama, Shoji; Yoshida, Mitsuteru; Kenzaki, Koichiro; Toba, Hiroaki; Kawakami, Yukikiyo; Okumura, Kazumasa; Takizawa, Hiromitsu; Kondo, Kazuya; Tangoku, Akira
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2016-08
• 出版年（W3CDTF）: 2016-08
• 並列タイトル等: マウス肺気腫モデルにおける胎仔肺移植による肺再生; Fetal tissue for mouse lung regeneration
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 63 3-4
• 掲載巻: 63
• 掲載号: 3-4
• 掲載ページ: 182-186
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2016-07-28
• 報告番号: 甲第2963号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Pallid mouse; COPD; lung regeneration; fetal tissue; pulmonary emphysema
• 対象利用者: 一般
• 一般注記: The mortality and morbidity of chronic obstructive pulmonary disease are high. However, no radical therapy has been developed to date. The purpose of this study was to evaluate whether fetal mouse lung tissue can grow and differentiate in the emphysematous lung. Fetal lung tissue from green fluorescent protein C57BL/6 mice at 16 days’ gestation was used as donor material. Twelve-month-old pallid mice were used as recipients. Donor lungs were cut into small pieces and implanted into the recipient left lung by performing thoracotomy under anesthesia. The recipient mice were sacrificed at day 7, 14, and 28 after implantation and used for histological examination. Well-developed spontaneous pulmonary emphysema was seen in 12-month-old pallid mice. Smooth and continuous connection between implanted fetal lung tissue and recipient lung was recognized. Air space expansion and donor tissue differentiation were observed over time. We could clearly distinguish the border zones between injected tissue and native tissue by the green fluorescence of grafts. Fetal mouse lung fragments survived and differentiated in the emphysematous lung of pallid mice. Implantation of fetal lung tissue in pallid mice might lead to further lung regeneration research from the perspective of respiratory and exercise function.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.63.182
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/111178/20190807111803836287/LID201610171001.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/111178/20190807111804155494/LID201610171002.pdf fulltext