Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker

• 資料種別: 博士論文
• タイトル: Flow Cytometric Evaluation of Surface CD56 Expression on Activated Natural Killer Cells as Functional Marker
• 著者・編者: Oboshi, Wataru; Aki, Kensaku; Tada, Tomoki; Watanabe, Toru; Yukimasa, Nobuyasu; Ueno, Ichiro; Saito, Ken; Hosoi, Eiji
• 著者標目: Oboshi, Wataru; Aki, Kensaku; Tada, Tomoki; Watanabe, Toru; Yukimasa, Nobuyasu; Ueno, Ichiro; Saito, Ken; Hosoi, Eiji
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2016-08
• 出版年（W3CDTF）: 2016-08
• 並列タイトル等: 機能的マーカーとしての活性化NK細胞表面CD56発現のフローサイトメトリー評価; Evaluation of Surface CD56 Expression
• タイトル（掲載誌）: The Journal of Medical Investigation
• 巻号年月日等（掲載誌）: 63 3-4
• 掲載巻: 63
• 掲載号: 3-4
• 掲載ページ: 199-203
• ISSN（掲載誌）: ISSN : 1349-6867; ISSN : 1343-1420
• 授与機関名: 徳島大学
• 授与年月日: 2016-09-15
• 報告番号: 甲第2975号
• 学位: 博士（保健学）
• 本文の言語コード: eng
• 件名標目: natural killer (NK) cells; CD56; cytotoxicity; IFN-γmRNA; flow cytometry
• 対象利用者: 一般
• 一般注記: Surface CD56 is the most important cell marker for defining NK cells. However, the relationship between the expression of surface CD56 and NK cell activity has not yet been elucidated in detail. Thirteen healthy volunteers were enrolled in the present study. Peripheral blood mononuclear cells (PBMCs) were stimulated with rIL-2 or rIL-12 (1, 10, 100 U/mL) for 18 h at 37 ℃. After incubation, surface CD56 expression on NK cells was evaluated using a flow cytometric analysis. A colorimetric-based lactate dehydrogenase (LDH) assay was used for experiments on cytotoxicity. IFN-γmRNA gene expression was quantified by real-time PCR. The expression level of surface CD56 on NK cells, cytotoxicity, and IFN-γmRNA gene expression were significantly increased by the rIL-2 and rIL-12 stimulations. In addition, a positive correlation was found between surface CD56 expression and cytotoxic activity or IFN-γmRNA gene expression. We revealed that the quantification of surface CD56 expression was applicable to the evaluation of cytotoxicity and IFN-γproduction in activated NK cells. These results suggest that the measurement of surface CD56 expression represent an easy and rapidly reproducible technique to evaluate the activated state of NK cells and monitor NK cell activity in immunotherapy.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.2152/jmi.63.199
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

https://repo.lib.tokushima-u.ac.jp/files/public/11/111181/20190729152853237523/LID201611141022.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111181/20190729152853972871/LID201611141023.pdf fulltext

https://repo.lib.tokushima-u.ac.jp/files/public/11/111181/20190729152854611837/LID201611141024.pdf fulltext