Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells

• 資料種別: 博士論文
• タイトル: Irradiation by ultraviolet light-emitting diodes inactivates influenza a viruses by inhibiting replication and transcription of viral RNA in host cells
• 著者・編者: Nishisaka-Nonaka, Risa; Mawatari, Kazuaki; Yamamoto, Tomomi; Kojima, Mizuki; Shimohata, Takaaki; Uebanso, Takashi; Nakahashi, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Wada, Takahiro; Okamoto, Masayuki; Ito, Hiroshi; Yoshida, Ken-ichi; Daidoji, Tomo; Nakaya, Takaaki; Takahashi, Akira
• 著者標目: Nishisaka-Nonaka, Risa; Mawatari, Kazuaki; Yamamoto, Tomomi; Kojima, Mizuki; Shimohata, Takaaki; Uebanso, Takashi; Nakahashi, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Wada, Takahiro; Okamoto, Masayuki; Ito, Hiroshi; Yoshida, Ken-ichi; Daidoji, Tomo; Nakaya, Takaaki; Takahashi, Akira
• 出版事項: Elsevier
• 出版年月日等: 2018-10-29
• 出版年（W3CDTF）: 2018-10-29
• 並列タイトル等: 紫外線発光ダイオード照射は宿主細胞内でのウイルスRNAの複製と転写を抑制することでA型インフルエンザウイルスを不活化する
• タイトル（掲載誌）: Journal of Photochemistry and Photobiology B: Biology
• 巻号年月日等（掲載誌）: 189
• 掲載巻: 189
• 掲載ページ: 193-200
• ISSN（掲載誌）: ISSN : 1011-1344
• 授与機関名: 徳島大学
• 授与年月日: 2020-03-23
• 報告番号: 甲第3415号
• 学位: 博士（栄養学）
• 本文の言語コード: eng
• 件名標目: Influenza A virus; ultraviolet; light-emitting diodes; UV-LED
• 対象利用者: 一般
• 一般注記: Influenza A viruses (IAVs) pose a serious global threat to humans and their livestock, especially poultry and pigs. This study aimed to investigate how to inactivate IAVs by using different ultraviolet-light-emitting diodes (UV-LEDs). We developed sterilization equipment with light-emitting diodes (LEDs) those peak wavelengths were 365 nm (UVA-LED), 310 nm (UVB-LED), and 280 nm (UVC-LED). These UV-LED irradiations decreased dose fluence-dependent plaque-forming units of IAV H1N1 subtype (A/Puerto Rico/8/1934) infected Madin-Darby canine kidney (MDCK) cells, but the inactivation efficiency of UVA-LED was significantly lower than UVB- and UVC-LED. UV-LED irradiations did not alter hemagglutination titer, but decreased accumulation of intracellular total viral RNA in infected MDCK cells was observed. Additionally, UV-LED irradiations suppressed the accumulation of intracellular mRNA (messenger RNA), vRNA (viral RNA), and cRNA (complementary RNA), as measured by strand-specific RT-PCR. These results suggest that UV-LEDs inhibit host cell replication and transcription of viral RNA. Both UVB- and UVC-LED irradiation decreased focus-forming unit (FFU) of H5N1 subtype (A/Crow/Kyoto/53/2004), a highly pathogenic avian IAV (HPAI), in infected MDCK cells, and the amount of FFU were lower than the H1N1 subtype. From these results, it appears that IAVs may have different sensitivity among the subtypes, and UVB- and UVC-LED may be suitable for HPAI virus inactivation.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.017
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/114608/2020041411132482388/k3415_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/114608/20200414111324631091/k3415_fulltext.pdf fulltext