Rho-associated protein kinase and cyclophilin a are involved in inorganic phosphate-induced calcification signaling in vascular smooth muscle cells

• 資料種別: 博士論文
• タイトル: Rho-associated protein kinase and cyclophilin a are involved in inorganic phosphate-induced calcification signaling in vascular smooth muscle cells
• 著者・編者: Tsuda, Tatsuya; Imanishi, Masaki; Oogoshi, Mizuho; Goda, Mitsuhiro; Kihira, Yoshitaka; Horinouchi, Yuya; Zamami, Yoshito; Ishizawa, Keisuke; Ikeda, Yasumasa; Hashimoto, Ichiro; Tamaki, Toshiaki; Izawa-Ishizawa, Yuki
• 著者標目: Tsuda, Tatsuya; Imanishi, Masaki; Oogoshi, Mizuho; Goda, Mitsuhiro; Kihira, Yoshitaka; Horinouchi, Yuya; Zamami, Yoshito; Ishizawa, Keisuke; Ikeda, Yasumasa; Hashimoto, Ichiro; Tamaki, Toshiaki; Izawa-Ishizawa, Yuki
• 出版事項: Japanese Pharmacological Society|Elsevier
• 出版年月日等: 2019-12-16
• 出版年（W3CDTF）: 2019-12-16
• 並列タイトル等: 血管平滑筋細胞石灰化シグナルにおけるRhoキナーゼ−サイクロフィリンA経路の関与
• タイトル（掲載誌）: Journal of Pharmacological Sciences
• 巻号年月日等（掲載誌）: 142 3
• 掲載巻: 142
• 掲載号: 3
• 掲載ページ: 109-115
• ISSN（掲載誌）: ISSN : 1347-8613
• 授与機関名: 徳島大学
• 授与年月日: 2022-02-24
• 報告番号: 甲第3572号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Arterial calcification; Cyclophilin A; Rho-associated protein kinase; Vascular smooth muscle cell; Atherosclerosis
• 対象利用者: 一般
• 一般注記: Arterial calcification, a risk factor of cardiovascular events, develops with differentiation of vascular smooth muscle cells (VSMCs) into osteoblast-like cells. Cyclophilin A (CypA) is a peptidyl-prolyl isomerase involved in cardiovascular diseases such as atherosclerosis and aortic aneurysms, and rho-associated protein kinase (ROCK) is involved in the pathogenesis of vascular calcification. CypA is secreted in a ROCK activity-dependent manner and works as a mitogen via autocrine or paracrine mechanisms in VSMCs. We examined the involvement of the ROCK-CypA axis in VSMC calcification induced by inorganic phosphate (Pi), a potent cell mineralization initiator. We found that Pi stimulated ROCK activity, CypA secretion, extracellular signal-regulated protein kinase (ERK) 1/2 phosphorylation, and runt-related transcription factor 2 expression, resulting in calcium accumulation in rat aortic smooth muscle cells (RASMCs). The ROCK inhibitor Y-27632 significantly suppressed Pi-induced CypA secretion, ERK1/2 phosphorylation, and calcium accumulation. Recombinant CypA was found to be associated with increased calcium accumulation in RASMCs. Based on these results, we suggest that autocrine CypA is mediated by ROCK activity and is involved in Pi-induced ERK1/2 phosphorylation following calcification signaling in RASMCs.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
• 関連情報（DOI）: 10.1016/j.jphs.2019.12.005
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/115530/20220407144329739307/k3572_abstract_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/115530/20220407144330193520/k3572_fulltext.pdf fulltext