Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells

• 資料種別: 博士論文
• タイトル: Fibroblast growth factor 2 suppresses the expression of C-C motif chemokine 11 through the c-Jun N-terminal kinase pathway in human dental pulp-derived mesenchymal stem cells
• 著者・編者: Kurogoushi, Rika; Hasegawa, Tomokazu; Akazawa, Yuki; Iwata, Kokoro; Sugimoto, Asuna; Yamaguchi-Ueda, Kimiko; Miyazaki, Aya; Narwidina, Anrizandy; Kawarabayashi, Keita; Kitamura, Takamasa; Nakagawa, Hiroshi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• 著者標目: Kurogoushi, Rika; Hasegawa, Tomokazu; Akazawa, Yuki; Iwata, Kokoro; Sugimoto, Asuna; Yamaguchi-Ueda, Kimiko; Miyazaki, Aya; Narwidina, Anrizandy; Kawarabayashi, Keita; Kitamura, Takamasa; Nakagawa, Hiroshi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• 出版事項: Spandidos Publications
• 出版年月日等: 2021-09-24
• 出版年（W3CDTF）: 2021-09-24
• 並列タイトル等: FGF2はヒト歯髄由来間葉系幹細胞においてJNK経路を介してCCL11の発現を抑制する; FGF2 SUPPRESSED CCL11 EXPRESSION IN HUMAN DENTAL PULP-DERIVED MSCs
• タイトル（掲載誌）: Experimental and Therapeutic Medicine
• 巻号年月日等（掲載誌）: 22 6
• 掲載巻: 22
• 掲載号: 6
• 掲載ページ: 1356-
• ISSN（掲載誌）: ISSN : 1792-0981; ISSN : 1792-1015
• 授与機関名: 徳島大学
• 授与年月日: 2022-03-23
• 報告番号: 甲第3615号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: dental pulp-derived mesenchymal stem cells; fibroblast growth factor 2; fibroblast growth factor receptor signal; C-C motif ligand 11; c-Jun N-terminal kinase
• 対象利用者: 一般
• 一般注記: The regulation of the mesenchymal stem cell (MSC) programming mechanism promises great success in regenerative medicine. Tissue regeneration has been associated not only with the differentiation of MSCs, but also with the microenvironment of the stem cell niche that involves various cytokines and immune cells in the tissue regeneration site. In the present study, fibroblast growth factor 2 (FGF2), the principal growth factor for tooth development, dental pulp homeostasis and dentin repair, was reported to affect the expression of cytokines in human dental pulp‑derived MSCs. FGF2 significantly inhibited the expression of chemokine C‑C motif ligand 11 (CCL11) in a time‑ and dose‑dependent manner in the SDP11 human dental pulp‑derived MSC line. This inhibition was diminished following treatment with the AZD4547 FGF receptor (FGFR) inhibitor, indicating that FGF2 negatively regulated the expression of CCL11 in SDP11 cells. Furthermore, FGF2 activated the phosphorylation of p38 mitogen‑activated protein kinase (p38 MAPK), extracellular signal‑regulated kinase 1/2 (ERK1/2) and c‑Jun N‑terminal kinases (JNK) in SDP11 cells. The mechanism of the FGFR‑downstream signaling pathway was then studied using the SB203580, U0126 and SP600125 inhibitors for p38 MAPK, ERK1/2, and JNK, respectively. Interestingly, only treatment with SP600125 blocked the FGF2‑mediated suppression of CCL11. The present results suggested that FGF2 regulated the expression of cytokines and suppressed the expression of CCL11 via the JNK signaling pathway in human dental pulp‑derived MSCs. The present findings could provide important insights into the association of FGF2 and CCL11 in dental tissue regeneration therapy.
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License. (https://creativecommons.org/licenses/by-nc-nd/4.0/)
• 関連情報（DOI）: 10.3892/etm.2021.10791
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/117039/20220517134120169492/k3615_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117039/20220517134120239190/k3615_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117039/20220517134120383801/k3615_fulltext.pdf fulltext