miR-144-3p/miR-451a promotes lymphovascular invasion through repression of PTEN/p19 in rectal neuroendocrine tumors

• 資料種別: 博士論文
• タイトル: miR-144-3p/miR-451a promotes lymphovascular invasion through repression of PTEN/p19 in rectal neuroendocrine tumors
• 著者・編者: Murayama, Noriaki; Okamoto, Koichi; Nakagawa, Tadahiko; Miyoshi, Jinsei; Nishida, Kensei; Kawaguchi, Tomoyuki; Kagemoto, Kaizo; Kitamura, Shinji; Ma, Beibei; Miyamoto, Hiroshi; Muguruma, Naoki; Yano, Mitsuyasu; Tsuneyama, Koichi; Fujimori, Takahiro; Sato, Yasushi; Takayama, Tetsuji
• 著者標目: Murayama, Noriaki; Okamoto, Koichi; Nakagawa, Tadahiko; Miyoshi, Jinsei; Nishida, Kensei; Kawaguchi, Tomoyuki; Kagemoto, Kaizo; Kitamura, Shinji; Ma, Beibei; Miyamoto, Hiroshi; Muguruma, Naoki; Yano, Mitsuyasu; Tsuneyama, Koichi; Fujimori, Takahiro; Sato, Yasushi; Takayama, Tetsuji
• 出版事項: Journal of Gastroenterology and Hepatology Foundation|Wiley
• 出版年月日等: 2022-03-24
• 出版年（W3CDTF）: 2022-03-24
• 並列タイトル等: 直腸神経内分泌腫瘍において、miR-144-3p/miR-451aはPTEN/p19を介して脈管侵襲を促進する
• タイトル（掲載誌）: Journal of Gastroenterology and Hepatology
• 巻号年月日等（掲載誌）: 37 5
• 掲載巻: 37
• 掲載号: 5
• 掲載ページ: 919-927
• ISSN（掲載誌）: ISSN : 1440-1746
• 授与機関名: 徳島大学
• 授与年月日: 2022-10-27
• 報告番号: 甲第3664号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: 直腸NET; miR-144-3p; miR-451a; PTEN; p19; rectal NET-G1; lymphovascular invasion; miR-144-3p/451a
• 対象利用者: 一般
• 一般注記: Background and Aim: Although rectal neuroendocrine tumor (NET-G1) have potential metastatic capability, even among small tumors, no predictive biomarker for invasion and metastasis has been reported. We analyzed microRNA (miRNA) expression profiles in rectal NET-G1 tissues with and without lymphovascular invasion (LVI). Moreover, we then investigated their target genes to clarify the mechanism of invasion/metastasis in NET-G1. Methods: miRNA array analysis was performed using 7 rectal NET-G1 tissues with LVI and 7 without LVI. miRNA expression was confirmed by quantitative real-time PCR. A NET cell line H727 was transfected with miRNA mimic or target gene small interfering RNA, and migration and invasion assays were performed. Results: The expression levels of miR-144-3p and miR-451a were significantly higher in NET-G1 with LVI versus without LVI, as determined by miRNA array analysis and RT-qPCR. A significant correlation was observed between miR-144-3p and miR-451a expression levels, strongly suggesting miR144/451 cluster overexpression in NET-G1 with LVI. Bioinformatic analysis of target genes revealed that miR-144-3p and miR-451a directly interact with PTEN and p19 mRNA, respectively. Immunohistochemistry revealed significantly lower expression of PTEN and p19 in NET-G1 tissues with LVI than in those without LVI. The miR-144-3p and miR-451a mimic significantly increased cell migration/invasion capability, respectively. Knockdown of PTEN and p19 induced significant augmentation of cell invasion and migration capability, respectively. Conclusions: Our data suggest that overexpression of miR-144/miR-451 cluster promotes LVI via repression of PTEN and p19 in rectal NET-G1 cells. miR-144/451 cluster may be a novel biomarker for predicting invasion/metastasis in rectal NET-G1.
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1111/jgh.15833
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/117699/20221111103546212310/k3664_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117699/20221111103546537085/k3664_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/117699/20221111131423355026/k3664_fulltext.pdf fulltext