Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression

• 資料種別: 博士論文
• タイトル: Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression
• 著者・編者: Narwidina, Anrizandy; Miyazaki, Aya; Iwata, Kokoro; Kurogoushi, Rika; Sugimoto, Asuna; Kudo, Yasusei; Kawarabayashi, Keita; Yamakawa, Yoshihito; Akazawa, Yuki; Kitamura, Takamasa; Nakagawa, Hiroshi; Yamaguchi-Ueda, Kimiko; Hasegawa, Tomokazu; Yoshizaki, Keigo; Fukumoto, Satoshi; Yamamoto, Akihito; Ishimaru, Naozumi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• 著者標目: Narwidina, Anrizandy; Miyazaki, Aya; Iwata, Kokoro; Kurogoushi, Rika; Sugimoto, Asuna; Kudo, Yasusei; Kawarabayashi, Keita; Yamakawa, Yoshihito; Akazawa, Yuki; Kitamura, Takamasa; Nakagawa, Hiroshi; Yamaguchi-Ueda, Kimiko; Hasegawa, Tomokazu; Yoshizaki, Keigo; Fukumoto, Satoshi; Yamamoto, Akihito; Ishimaru, Naozumi; Iwasaki, Tomonori; Iwamoto, Tsutomu
• 出版事項: Elsevier
• 出版年月日等: 2023-02-04
• 出版年（W3CDTF）: 2023-02-04
• 並列タイトル等: ホメオボックス型転写因子Irx3はWnt5aの発現に関与し象牙芽細胞の増殖と分化に調整する
• タイトル（掲載誌）: Biochemical and Biophysical Research Communications
• 巻号年月日等（掲載誌）: 650
• 掲載巻: 650
• 掲載ページ: 47-54
• ISSN（掲載誌）: ISSN : 0006-291X; ISSN : 1090-2104
• 授与機関名: 徳島大学
• 授与年月日: 2023-09-20
• 報告番号: 甲第3748号
• 学位: 博士（歯学）
• 本文の言語コード: eng
• 件名標目: Iroquois homeobox genes; Irx3; Odontoblasts; Cell proliferation; Cell differentiation; Wnt5a
• 対象利用者: 一般
• 一般注記: Iroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal day 1 (P1) tooth germs, we examined the expression of all Irx genes (Irx1-Irx6) by RT-PCR and found that all genes except Irx4 were expressed in the tooth tissue. Irx1-Irx3 a were expressed in the dental epithelial cell line M3H1 cells, while Irx3 and Irx5 were expressed in the dental mesenchymal cell line mDP cells. Only Irx3 was expressed in both undifferentiated cell lines. Immunostaining also revealed the presence of IRX3 in the dental epithelial cells and mesenchymal condensation. Inhibition of endogenous Irx3 by siRNA blocks the proliferation and differentiation of mDP cells. Wnt3a, Wnt5a, and Bmp4 are factors involved in odontoblast differentiation and were highly expressed in mDP cells by quantitative PCR analysis. Interestingly, the expression of Wnt5a (but not Wnt3a or Bmp4) was suppressed by Irx3 siRNA. These results suggest that Irx3 plays an essential role in part through the regulation of Wnt5a expression during odontoblast proliferation and differentiation.
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
• 関連情報（DOI）: 10.1016/j.bbrc.2023.02.004
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/118052/20231018111816579771/k3748_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118052/20231018111816740995/k3748_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118052/20231018111816878084/k3748_fulltext.pdf fulltext