Blue light induces apoptosis and autophagy by promoting ROS-mediated mitochondrial dysfunction in synovial sarcoma

• 資料種別: 博士論文
• タイトル: Blue light induces apoptosis and autophagy by promoting ROS-mediated mitochondrial dysfunction in synovial sarcoma
• 著者・編者: Takeuchi, Makoto; Nishisho, Toshihiko; Toki, Shunichi; Kawaguchi, Shinji; Tamaki, Shunsuke; Oya, Takeshi; Uto, Yoshihiro; Katagiri, Toyomasa; Sairyo, Koichi
• 著者標目: Takeuchi, Makoto; Nishisho, Toshihiko; Toki, Shunichi; Kawaguchi, Shinji; Tamaki, Shunsuke; Oya, Takeshi; Uto, Yoshihiro; Katagiri, Toyomasa; Sairyo, Koichi
• 出版事項: John Wiley & Sons
• 出版年月日等: 2023-02-01
• 出版年（W3CDTF）: 2023-02-01
• 並列タイトル等: 青色光は滑膜肉腫に対して活性酸素種によるミトコンドリア機能障害を起こし、アポトーシスとオートファジーを誘導する
• タイトル（掲載誌）: Cancer Medicine
• 巻号年月日等（掲載誌）: 12 8
• 掲載巻: 12
• 掲載号: 8
• 掲載ページ: 9668-9683
• ISSN（掲載誌）: ISSN : 2045-7634
• 授与機関名: 徳島大学
• 授与年月日: 2023-03-23
• 報告番号: 甲第3704号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: synovial sarcoma; blue light; reactive oxygen species; apoptosis; autophagy; mitochondria
• 対象利用者: 一般
• 一般注記: Background: Synovial sarcoma (SS) has limited treatment options and there is an urgent need to develop a novel therapeutic strategy to treat SS. Blue light (BL) has been shown to inhibit the growth of several cancer cells. However, the efficacy of BL in soft tissue sarcomas such as SS has not been demonstrated, and the detailed mechanism underlying the antitumor activity of BL is not fully understood. In this study, we investigated the antitumor effect of BL on SS. Methods: Human SS cell lines were continuously irradiated with BL using light-emitting diodes (LEDs) in an incubator for in vitro analysis. The chicken chorioallantoic membrane (CAM) tumors and xenograft tumors in mice were subjected to daily BL irradiation with LEDs. Results: BL caused growth inhibition of SS cells and histological changes in CAM tumors. BL also suppressed the migration and invasion abilities of SS cells. The type of cell death in SS cells was revealed to be apoptosis. Furthermore, BL induced excessive production of reactive oxygen species (ROS) in mitochondria, resulting in oxidative stress and malfunctioned mitochondria. Reducing the production of ROS using N-acetylcysteine (NAC), a ROS scavenger, attenuated the inhibitory effect of BL on SS cells and mitochondrial dysfunction. In addition, BL induced autophagy, which was suppressed by the administration of NAC. The autophagy inhibitor of 3-methyladenine and small interfering RNA against the autophagy marker light chain 3B facilitated apoptotic cell death. Moreover, BL suppressed tumor growth in a mouse xenograft model. Conclusion: Taken together, our results revealed that BL induced apoptosis via the ROS-mitochondrial signaling pathway, and autophagy was activated in response to the production of ROS, which protected SS cells from apoptosis. Therefore, BL is a promising candidate for the development of an antitumor therapeutic strategy targeting SS.
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
• 関連情報（DOI）: 10.1002/cam4.5664
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 徳島大学 : 徳島大学機関リポジトリ

目次

http://repo.lib.tokushima-u.ac.jp/files/public/11/118302/2023060709564018556/k3704_abstract.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118302/20230607095640324614/k3704_review.pdf fulltext

http://repo.lib.tokushima-u.ac.jp/files/public/11/118302/20230607095640599132/k3704_fulltext.pdf fulltext