一般注記Large-scale cohort studies using saliva samples have been performed to elucidate the relationship between oral and systemic health and oral resident microbiota. Although standardization of the sampling and processing procedures for saliva that is used in next-generation sequencing (NGS) analysis is underway, the storage conditions after collecting saliva have not been studied in detail. In this study, we monitored changes in the salivary microbiota during short-term storage at room temperature. Saliva samples were collected from 11 subjects and stored at room temperature (23°C) for 3 hours. When the V3–V4 region of the 16S rRNA gene was analyzed using NGS, there was no change in the β-diversity of salivary bacterial microbiota, but a significant decrease in α-diversity was observed. Additionally, a significant decrease was observed in four operational taxonomic units (OTUs). These results suggest that even short-term storage of saliva samples at room temperature may affect the diversity of salivary microbiota and the relative abundance of specific OTUs.
2019年度
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース(IRDB)(機関リポジトリ)