並列タイトル等脳梗塞急性期の咀嚼機能が中枢神経再生能におよぼす影響
一般注記We examined the influence of masticatory function on central nervous system regeneration after the onset of cerebral infarction. For this experiment, we used a CB17 mouse cerebral ischemia model, which has high reproducibility. The mice were divided into two groups: the hard diet group (the left middle cerebral artery was occluded, and hard, solid food was given after the ischemic operation), and the soft diet group (the left middle cerebral artery was occluded, the upper and lower incisors were extracted, and powdery food was given after the ischemic operation). Brain tissue sections were prepared in both groups at 3, 7 and 14 days after the ischemic operation, and the pattern of nerve, glial and neural stem cell expression was investigated using immunohistochemical staining. In the hard diet group, although the expression of astrocytes and nestin-positive neural stem cells was confirmed in infarcted foci up to 7 days after the ischemic operation, there was no expression of astrocytes or neural stem cells in infarcted foci at 14 days after the operation. On the other hand, in the soft diet group, although the expression of astrocytes was confirmed in infarcted foci up to 14 days after the ischemic operation, nestin-positive neural stem cells were confirmed in infarcted foci 3 days after ischemia. In the penumbra area, neural stem cell expression was noted in the two groups, suggesting that the nerve regeneration capacity was maintained. In the hippocampal dentate gyrus, nestin-positive and GFAP-positive cells were observed up to 14 days after ischemia in both groups. In the hard diet group, the expressions of nestin-positive and GFAP-positive cells reached a peak at 14 days after the ischemic operation, confirming the enhancement of reactive astrocyte expression. On the other hand, in the soft diet group, the expressions of nestin-positive and GFAP-positive cells reached a peak at 7 days after the ischemic operation. The results of this experiment confirmed that intrinsic neural stem cells were induced in the infarcted area and hippocampal dentate gyrus in the acute phase of cerebral infarction, suggesting that the nerve regeneration capacity in the hippocampal dentate gyrus is enhanced during the early phase in the presence of masticatory hypofunction, in comparison with a state in which the masticatory function is maintained. In addition, we found that reactive astrocytes were closely involved in the mechanism of nerve regeneration in the hippocampal area.
2021年度
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）