一般注記Natural aldolase enzymes and created retro-aldolase protein catalysts often catalyze both aldol and retro-aldol reactions depending on the concentrations of the reactants and the products. Here, we report that the directionality of protein catalysts can be altered by replacing one amino acid. The protein catalyst derived from a scaffold of a previously reported retro-aldolase catalyst, catalyzed aldol reactions more efficiently than the previously reported retro-aldolase catalyst. The retro-aldolase catalyst efficiently catalyzed the retro-aldol reaction but was less efficient in catalyzing the aldol reaction. The results indicate that protein catalysts with varying levels of directionality in usually reversibly catalyzed aldol and retro-aldol reactions can be generated from the same protein scaffold.
source:https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202100435
一次資料へのリンクURLhttps://oist.repo.nii.ac.jp/?action=repository_action_common_download&item_id=2354&item_no=1&attribute_id=22&file_no=1
著作権情報© 2021 Wiley-VCH. This is the peer reviewed version of the following article: Fujioka, T., Numoto, N., Akama, H., Shilpa, K., Oka, M., Roy, P.K., Krishna, Y., Ito, N., Baker, D., Oda, M. and Tanaka, F. (2021), Varying the Directionality of Protein Catalysts for Aldol and Retro-Aldol Reactions. ChemBioChem. https://doi.org/10.1002/cbic.202100435, which has been published in final form at https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202100435. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）