Involvement of autoimmunity to REG, a regeneration factor, in patients with primary Sjögren's syndrome

• 資料種別: 博士論文
• タイトル: Involvement of autoimmunity to REG, a regeneration factor, in patients with primary Sjögren's syndrome
• 著者・編者: Yoshimoto, Kiyomi; Fujimoto, Takashi; Itaya-Hironaka, Asako; Miyaoka, Tomoko; Sakuramoto-Tsuchida, Sumiyo; Yamauchi, Akiyo; Takeda, Maiko; Kasai, Takahiko; Nakagawara, Kan-ichi; Nonomura, Akitaka; Takasawa, Shin
• 著者標目: Yoshimoto, Kiyomi; Fujimoto, Takashi; Itaya-Hironaka, Asako; Miyaoka, Tomoko; Sakuramoto-Tsuchida, Sumiyo; Yamauchi, Akiyo; Takeda, Maiko; Kasai, Takahiko; Nakagawara, Kan-ichi; Nonomura, Akitaka; Takasawa, Shin
• 出版事項: Blackwell Scientific Pub.
• 出版年月日等: 2013-10
• 出版年（W3CDTF）: 2013-10
• 並列タイトル等: 原発性シェーグレン症候群患者における再生因子REG(regenerating gene)に対する自己免疫の関与
• タイトル（掲載誌）: Clinical and experimental immunology
• 巻号年月日等（掲載誌）: 174 1
• 掲載巻: 174
• 掲載号: 1
• 掲載ページ: 1-9
• ISSN（掲載誌）: ISSN : 0009-9104
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2013-11-27
• 報告番号: 乙第1319号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: REG family genes; REG Iα protein; anti-REG Iα autoantibodies; Sjögren’s syndrome; ductal epithelial cells
• 対象利用者: 一般
• 一般注記: type:Thesis; The regenerating gene (Reg) was isolated originally as a gene specifically over-expressed in regenerating pancreatic islets and constitute a growth factor family. Reg gene product (Reg) is important in the pathophysiology of various human inflammatory diseases. Recently, the possible involvement of human REG in the regeneration of salivary ductal epithelial cells of patients with primary Sjögren's syndrome (SS) was reported. However, the expression of the REG family genes in minor salivary glands (MSG) and the occurrence of anti-REG Iα autoantibodies in SS patients were obscured. In this study, we examined the expression of REG family genes in the MSG of SS and screened anti-REG Iα autoantibodies in SS. The mRNA levels of REG family genes in MSG were quantified using real-time reverse transcription-polymerase chain reaction (RT-PCR) and REG Iα expression in the MSG was analysed by immunohistochemistry. The mRNA level of REG Iα in the MSG of SS patients was significantly higher than that of control. REG Iα protein was expressed highly in SS ductal epithelial cells. Anti-REG Iα autoantibodies in the sera were found in 11% of SS. All the MSG in the anti-REG Iα autoantibody-positive group showed REG Iα expression, whereas only 40% showed REG Iα expression in the anti-REG Iα autoantibody-negative group. The anti-REG Iα autoantibody-positive group showed significantly lower saliva secretion and a higher ratio of grade 4 (by Rubin-Holt) in sialography. These data suggest strongly that autoimmunity to REG Iα might play a role in the degeneration of MSG ductal epithelial cells in primary SS.; 博士（医学）・乙第1319号・平成25年11月27日; The definitive version is available at " http://dx.doi.org/10.1111/cei.12142 "; identifier:Clinical and experimental immunology Vol.174 No.1 p.1-9; identifier:00099104; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/2674; identifier:Clinical and experimental immunology, 174(1): 1-9
• 一次資料へのリンクURL: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2674/1/01%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2674/2/02%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2674/6/03%e6%9c%ac%e6%96%87.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/2674/7/04Figure_1-4.pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1111/cei.12142
• 掲載誌（NCID）: AA00607716; https://ci.nii.ac.jp/ncid/AA00607716
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU