Syndecan-1 responsive microRNA-126 and 149 regulate cell proliferation in prostate cancer.

• 資料種別: 博士論文
• タイトル: Syndecan-1 responsive microRNA-126 and 149 regulate cell proliferation in prostate cancer.
• 著者・編者: Fujii, Tomomi; Shimada, Keiji; Tatsumi, Yoshihiro; Fujimoto, Kiyohide; Konishi, Noboru; 藤井, 智美; 島田, 啓司; 辰巳, 佳弘; 藤本, 清秀; 小西, 登
• 著者標目: Fujii, Tomomi; Shimada, Keiji; Tatsumi, Yoshihiro; Fujimoto, Kiyohide; Konishi, Noboru; 藤井, 智美; 島田, 啓司; 辰巳, 佳弘; 藤本, 清秀; 小西, 登
• 出版事項: Elsevier
• 出版年月日等: 2015-01
• 出版年（W3CDTF）: 2015-01
• 並列タイトル等: Syndecan-1を介したmicroRNA-126および149は前立腺癌における細胞増殖を制御する
• タイトル（掲載誌）: Biochemical and biophysical research communications
• 巻号年月日等（掲載誌）: 456 1
• 掲載巻: 456
• 掲載号: 1
• 掲載ページ: 183-189
• ISSN（掲載誌）: ISSN : 0006-291X
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2015-05-28
• 報告番号: 乙第1358号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: MicroRNA; Syndecan-1; MiR-126; MiR-149; Prostate cancer
• 対象利用者: 一般
• 一般注記: type:Thesis; MicroRNAs (miRNAs) are short (19–24 nt), low molecular weight RNAs that play important roles in the regulation of target genes associated with cell proliferation, differentiation, and development, by binding to the 3′-untranslated region of the target mRNAs. In this study, we examined the expression of miRNA-126 (miR-126) and miR-149 in prostate cancer, and investigated the molecular mechanisms by which they affect syndecan-1 in prostate cancer. Functional analysis of miR-126 and miR-149 was conducted in the prostate cancer cell lines, PC3, Du145, and LNCaP. The expression levels of SOX2, NANOG, Oct4, miR-126 and miR-149 were evaluated by quantitative RT-PCR. After silencing syndecan-1, miR-126, and/or miR-149 in the PC3 cells, cell proliferation, senescence, and p21 induction were assessed using the MTS assay, senescence-associated β-galactosidase (SA-β-Gal) assay, and immunocytochemistry, respectively. Compared to the Du145 and LNCaP cells, PC3 cells exhibited higher expression of syndecan-1. When syndecan-1 was silenced, the PC3 cells showed reduced expression of miR-126 and miR-149 most effectively. Suppression of miR-126 and/or miR-149 significantly inhibited cell growth via p21 induction and subsequently, induced senescence. The mRNA expression levels of SOX2, NANOG, and Oct4 were significantly increased in response to the silencing of miR-126 and/or miR-149. Our results suggest that miR-126 and miR-149 are associated with the expression of syndecan-1 in prostate cancer cells. These miRNAs promote cell proliferation by suppressing SOX2, NANOG, and Oct4. The regulation of these factors by miR-126 and miR-149 is essential for syndecan-1-mediated development of androgen-refractory prostate cancer.; 博士（医学）・乙第1358号・平成27年5月28日; Copyright © 2014 Elsevier Inc.; identifier:Biochemical and biophysical research communications Vol.456 No.1 p.183-189(2015.01); identifier:0006291X; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3071; identifier:Biochemical and biophysical research communications, 456(1): 183-189
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1016/j.bbrc.2014.11.056
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3071/1/01_%e4%b9%991358%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3071/2/02_%e4%b9%991358%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3071/6/03_%e4%b9%991358%e6%9c%ac%e6%96%87.pdf fulltext