Promotion of osteogenesis and angiogenesis in vascularized tissue-engineered bone using osteogenic matrix cell sheets.

• 資料種別: 博士論文
• タイトル: Promotion of osteogenesis and angiogenesis in vascularized tissue-engineered bone using osteogenic matrix cell sheets.
• 著者・編者: Nakano, Kenichi; Murata, Keiichi; Omokawa, Shohei; Akahane, Manabu; Shimizu, Takamasa; Kawamura, Kenji; Kawate, Kenji; Tanaka, Yasuhito
• 著者標目: Nakano, Kenichi; Murata, Keiichi; Omokawa, Shohei; Akahane, Manabu; Shimizu, Takamasa; Kawamura, Kenji; Kawate, Kenji; Tanaka, Yasuhito
• 出版事項: Lippincott Williams & Wilkins
• 出版年月日等: 2016
• 出版年（W3CDTF）: 2016
• 並列タイトル等: 骨形成細胞シートは血管柄付き人工骨内での骨形成および血管形成を促進させる
• タイトル（掲載誌）: Plastic and reconstructive surgery
• 巻号年月日等（掲載誌）: 2016(掲載予定)
• 掲載巻: 2016(掲載予定)
• ISSN（掲載誌）: ISSN : 0032-1052
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2016-03-15
• 報告番号: 甲第652号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: type:Thesis; BACKGROUND: The regeneration of large, poorly vascularized bone defects remains a significant challenge. Although vascularized bone grafts promote osteogenesis, the required tissue harvesting causes problematic donor-site morbidity. Artificial bone substitutes are promising alternatives for regenerative medicine applications, but the incorporation of suitable cells and/or growth factors is necessary for their successful clinical application. The inclusion of vascular bundles can further enhance the bone-forming capability of bone substitutes by promoting tissue neovascularization. Little is known about how neovascularization occurs and how new bone extends within vascularized tissue-engineered bone, because no previous studies have used tissue-engineered bone to treat large, poorly vascularized defects. METHODS: In this study, the authors developed a novel vascularized tissue-engineered bone scaffold composed of osteogenic matrix cell sheets wrapped around vascular bundles within β-tricalcium phosphate ceramics. RESULTS: Four weeks after subcutaneous transplantation in rats, making use of the femoral vascular bundle, vascularized tissue-engineered bone demonstrated more angiogenesis and higher osteogenic potential than the controls. After vascularized tissue-engineered bone implantation, abundant vascularization and new bone formation were observed radially from the vascular bundle, with increased mRNA expression of alkaline phosphatase, bone morphogenetic protein-2, osteocalcin, and vascular endothelial growth factor-A. CONCLUSION: This novel method for preparing vascularized tissue-engineered bone scaffolds may promote the regeneration of large bone defects, particularly where vascularization has been compromised.; 博士（医学）・甲第652号・平成28年3月15日; Copyright © 2016 American Society of Plastic Surgeons All rights reserved.; This is a non-final version of an article published in final form in "http://dx.doi.org/10.1097/PRS.0000000000002079"; identifier:Plastic and reconstructive surgery Vol.137 No.5 p.1476-1484(2016 May); identifier:00321052; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3190; identifier:Plastic and reconstructive surgery, 2016（掲載予定）
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3190/1/01_%e7%94%b2652%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3190/2/02_%e7%94%b2652%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3190/10/03_%e7%94%b2652%e6%9c%ac%e6%96%87.pdf fulltext