Effectiveness of Bone Marrow Stromal Cell Sheets in Maintaining Random-Pattern Skin Flaps in an Experimental Animal Model.

• 資料種別: 博士論文
• タイトル: Effectiveness of Bone Marrow Stromal Cell Sheets in Maintaining Random-Pattern Skin Flaps in an Experimental Animal Model.
• 著者・編者: Kira, Tsutomu; Omokawa, Shohei; Akahane, Manabu; Shimizu, Takamasa; Nakano, Kenichi; Nakanishi, Yasuaki; Onishi, Tadanobu; Kido, Akira; Inagaki, Yusuke; Tanaka, Yasuhito
• 著者標目: Kira, Tsutomu; Omokawa, Shohei; Akahane, Manabu; Shimizu, Takamasa; Nakano, Kenichi; Nakanishi, Yasuaki; Onishi, Tadanobu; Kido, Akira; Inagaki, Yusuke; Tanaka, Yasuhito
• 出版事項: Lippincott Williams & Wilkins
• 出版年月日等: 2015-11
• 出版年（W3CDTF）: 2015-11
• 並列タイトル等: 動物モデルにおける骨髄間質細胞シートの乱軸型皮弁の延長効果
• タイトル（掲載誌）: Plastic and reconstructive surgery
• 巻号年月日等（掲載誌）: 136 5
• 掲載巻: 136
• 掲載号: 5
• 掲載ページ: 624e-632e
• ISSN（掲載誌）: ISSN : 0032-1052
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2016-11-24
• 報告番号: 甲第658号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: type:Thesis; BACKGROUND: Bone marrow stromal cells can be applied therapeutically to enhance angiogenesis; however, the use of bone marrow stromal cell suspensions reduces efficiency because of low-level attachment. The authors hypothesized that bone marrow stromal cell sheets would facilitate cell fixation, thus enhancing angiogenesis. The authors investigated flap survival area and enhancement of angiogenic factors in a rat random-pattern skin flap model after application of bone marrow stromal cell sheets. METHODS: Bone marrow stromal cell sheets (prepared from 7-week-old rat femurs) were cultured under four different hypoxic conditions. Sheets with the highest angiogenic potential, determined by an in vitro pilot study, were injected into subcutaneous layers of the rat dorsum (bone marrow stromal cell sheet group). A control group (phosphate-buffered saline only) was included. On day 2 after injection, caudally based random-pattern skin flaps (12 × 3 cm) were elevated. On day 7 after elevation, surviving skin flap areas were measured. Skin samples were harvested from each flap and gene expression levels of vascular endothelial growth factor and basic fibroblast growth factor were measured by quantitative real-time polymerase chain reaction. RESULTS: Skin flap survival area (71.6 ± 2.3 percent versus 51.5 ± 3.3 percent) and levels of vascular endothelial growth factor and basic fibroblast growth factor were significantly higher in the bone marrow stromal cell sheet group than in the control group (p < 0.05). CONCLUSIONS: Implantation of bone marrow stromal cell sheets increased the survival area of random-pattern skin flaps. Expression of angiogenic factors may have contributed to the increased flap survival.; 博士（医学）・甲第658号・平成28年11月24日; Copyright © 2015 American Society of Plastic Surgeons; The definitive version is available at " http://dx.doi.org/10.1097/PRS.0000000000001679 "; identifier:Plastic and reconstructive surgery Vol.136 No.5 p.624e-632e(2015 Nov); identifier:00321052; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3284; identifier:Plastic and reconstructive surgery, 136(5): 624e-632e
• 一次資料へのリンクURL: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3284/1/01_%e7%94%b2658%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3284/2/02_%e7%94%b2658%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3284/3/03_%e7%94%b2658%e6%9c%ac%e6%96%87.pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1097/PRS.0000000000001679
• 掲載誌（NCID）: AA00775696; https://ci.nii.ac.jp/ncid/AA00775696
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU