Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.

• 資料種別: 博士論文
• タイトル: Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.
• 著者・編者: Matsunari, Yasunori; Sugimoto, Mitsuhiko; Doi, Masaaki; Matsui, Hideto; Kawaguchi, Masahiko
• 著者標目: Matsunari, Yasunori; Sugimoto, Mitsuhiko; Doi, Masaaki; Matsui, Hideto; Kawaguchi, Masahiko
• 出版事項: Springer Nature
• 出版年月日等: 2016-12
• 出版年（W3CDTF）: 2016-12
• 並列タイトル等: 全血流動下でのフォンビルブランド因子依存性血栓形成における組織因子の機能特性
• タイトル（掲載誌）: International journal of hematology
• 巻号年月日等（掲載誌）: 104 6
• 掲載巻: 104
• 掲載号: 6
• 掲載ページ: 661-668
• ISSN（掲載誌）: ISSN : 0925-5710
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2016-11-24
• 報告番号: 乙第1388号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Blood flow; Fibrin; Thrombus formation; Tissue factor; Von Willebrand factor
• 対象利用者: 一般
• 一般注記: type:Thesis; Von Willebrand factor (VWF) plays an important role in mediating platelet adhesion and aggregation under high shear rate conditions. Such platelet aggregates are strengthened by fibrin-network formation triggered by tissue factor (TF). However, little is known about the role of TF in VWF-dependent thrombus formation under blood flow conditions. We evaluated TF in thrombus formation on immobilized VWF under whole blood flow conditions in an in vitro perfusion chamber system. Surface-immobilized TF amplified intra-thrombus fibrin generation significantly under both low and high shear flow conditions, while TF in sample blood showed no appreciable effects. Furthermore, immobilized TF enhanced VWF-dependent platelet adhesion and aggregation significantly under high shear rates. Neutrophil cathepsin G and elastase increased significantly intra-thrombus fibrin deposition on immobilized VWF–TF complex, suggesting the involvement of leukocyte inflammatory responses in VWF/TF-dependent mural thrombogenesis under these flow conditions. These results reveal a functional link between VWF and TF under whole blood flow conditions, in which surface-immobilized TF and VWF mutually contribute to mural thrombus formation, which is essential for normal hemostasis. By contrast, TF circulating in blood may be involved in systemic hypercoagulability, as seen in sepsis caused by severe microbial infection, in which neutrophil inflammatory responses may be active.; 博士（医学）・乙第1388号・平成28年11月24日; © The Japanese Society of Hematology 2016; The original publication is available at www.springerlink.com; The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-016-2086-z; identifier:International journal of hematology Vol.104 No.6 p.661-668 (2016 Dec); identifier:09255710; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3293; identifier:International journal of hematology, 104(6): 661-668
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1007/s12185-016-2086-z
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/1/01_%e4%b9%991388%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/2/02_%e4%b9%991388%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/4/03_%e4%b9%991388%e6%9c%ac%e6%96%87.pdf fulltext