Topical and systemic immunoreaction triggered by intravesical chemotherapy in an N-butyl-N-(4-hydroxybutyl) nitorosamine induced bladder cancer mouse model.

• 資料種別: 博士論文
• タイトル: Topical and systemic immunoreaction triggered by intravesical chemotherapy in an N-butyl-N-(4-hydroxybutyl) nitorosamine induced bladder cancer mouse model.
• 著者・編者: Hori, Shunta; Miyake, Makito; Tatsumi, Yoshihiro; Onishi, Sayuri; Morizawa, Yosuke; Nakai, Yasushi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• 著者標目: Hori, Shunta; Miyake, Makito; Tatsumi, Yoshihiro; Onishi, Sayuri; Morizawa, Yosuke; Nakai, Yasushi; Tanaka, Nobumichi; Fujimoto, Kiyohide
• 出版事項: Public Library of Science
• 出版年月日等: 2017-04-13
• 出版年（W3CDTF）: 2017-04-13
• 並列タイトル等: N-ブチル-N-（4-ヒドロキシブチル）ニトロソアミン誘発膀胱癌マウスモデルを用いた膀胱内化学療法による局所及び全身の免疫応答
• タイトル（掲載誌）: PloS one
• 巻号年月日等（掲載誌）: 12 4
• 掲載巻: 12
• 掲載号: 4
• 掲載ページ: Article No.e0175494-
• ISSN（掲載誌）: ISSN : 1932-6203
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2019-03-15
• 報告番号: 甲第695号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 対象利用者: 一般
• 一般注記: type:Thesis; Intravesical bacillus Calmette-Guerin (BCG) treatment is the most common therapy to prevent progression and recurrence of non-muscle invasive bladder cancer (NMIBC). Although the immunoreaction elicited by BCG treatment is well documented, those induced by intravesical treatment with chemotherapeutic agents are much less known. We investigated the immunological profiles caused by mitomycin C, gemcitabine, adriamycin and docetaxel in the N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced orthotopic bladder cancer mouse model. Ninety mice bearing orthotopic bladder cancer induced by BBN were randomly divided into six groups and treated with chemotherapeutic agents once a week for four weeks. After last treatment, bladder and serum samples were analyzed for cell surface and immunological markers (CD4, CD8, CD56, CD204, Foxp3, and PD-L1) using immunohistochemistry staining. Serum and urine cytokine levels were evaluated by ELISA. All chemotherapeutic agents presented anti-tumor properties similar to those of BCG. These included changes in immune cells that resulted in fewer M2 macrophages and regulatory T cells around tumors. This result was compatible with those in human samples. Intravesical chemotherapy also induced systemic changes in cytokines, especially urinary interleukin (IL)-17A and granulocyte colony stimulating factor (G-CSF), as well as in the distribution of blood neutrophils, lymphocytes, and monocytes. Our findings suggest that intravesical treatment with mitomycin C and adriamycin suppresses protumoral immunity while enhancing anti-tumor immunity, possibly through the action of specific cytokines. A better understanding of the immunoreaction induced by chemotherapeutic agents can lead to improved outcomes and fewer side effects in intravesical chemotherapy against NMIBC.; 博士（医学）・甲第695号・平成31年3月15日; Copyright: © 2017 Hori et al. This is an open access article distributed under the terms of the Creative Commons Attribution License(https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.; identifier:PloS one Vol.12 No.4 Article No.e0175494 (2017 Apr); identifier:19326203; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3543; identifier:PloS one, 12(4): Article No.e0175494
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1371/journal.pone.0175494
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3543/1/01%e7%94%b2695%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3543/2/02%e7%94%b2695%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3543/3/03%e7%94%b2695%e6%9c%ac%e6%96%87.pdf fulltext