Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament.

• 資料種別: 博士論文
• タイトル: Silicate-substituted strontium apatite nano coating improves osteogenesis around artificial ligament.
• 著者・編者: Egawa, Takuya; Inagaki, Yusuke; Akahane, Manabu; Furukawa, Akira; Inoue, Kazuya; Ogawa, Munehiro; Tanaka, Yasuhito
• 著者標目: Egawa, Takuya; Inagaki, Yusuke; Akahane, Manabu; Furukawa, Akira; Inoue, Kazuya; Ogawa, Munehiro; Tanaka, Yasuhito
• 出版事項: BioMed Central
• 出版年月日等: 2019-08-31
• 出版年（W3CDTF）: 2019-08-31
• 並列タイトル等: 人工靭帯へのケイ酸ストロンチウムアパタイトナノコートは骨形成を促進する
• タイトル（掲載誌）: BMC musculoskeletal disorders
• 巻号年月日等（掲載誌）: 20 1
• 掲載巻: 20
• 掲載号: 1
• 掲載ページ: Article No.396-
• ISSN（掲載誌）: ISSN : 1471-2474
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2019-12-05
• 報告番号: 甲第724号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Silicate-substituted strontium; Anterior cruciate ligament injuries; Nano coating; Osteogenesis; PET artificial ligament
• 対象利用者: 一般
• 一般注記: 出版タイプ： VoR; type:Thesis; Background: Treatment of anterior cruciate ligament injuries commonly involves the use of polyethylene terephthalate (PET) artificial ligaments for reconstruction. However, the currently available methods require long fixation periods, thereby necessitating the development of alternative methods to accelerate the healing process between tendons and bones. Thus, we developed and evaluated a novel technique that utilizes silicate-substituted strontium (SrSiP). Methods: PET films, nano-coated with SrSiP, were prepared. Bone marrow mesenchymal cells (BMSCs) from femurs of male rats were cultured and seeded at a density of 1.0 × 104/cm2 onto the SrSiP-coated and non-coated PET film, and subsequently placed in an osteogenic medium. The osteocalcin concentration secreted into the medium was compared in each case. Next, PET artificial ligament, nano-coated with SrSiP, were prepared. BMSCs were seeded at a density of 4.5 × 105/cm2 onto the SrSiP-coated, and non-coated artificial ligament, and then placed in osteogenic medium. The osteocalcin and calcium concentrations in the culture medium were measured on the 8th, 10th, 12th, and 14th day of culture. Furthermore, mRNA expression of osteocalcin, alkaline phosphatase (ALP), bone morphogenetic protein-2 (BMP2), and runt-related transcription factor 2 (Runx2) was evaluated by qPCR. We transplanted the SrSiP-coated and non-coated artificial ligament to the tibiae of mature New Zealand white rabbits. Two months later, we sacrificed them and histologically evaluated them. Results: The secretory osteocalcin concentration in the medium on the film was significantly higher for the SrSiP group than for the non-coated group. Secretory osteocalcin concentration in the medium on the artificial ligament was also significantly higher in the SrSiP group than in the non-coated group on the 14th day. Calcium concentration on the artificial ligament was significantly lower in the SrSiP group than in the non-coated group on the 8th, 10th, 12th, and 14th day. In qPCR as well, OC, ALP, BMP2, and Runx2 mRNA expression were significantly higher in the SrSiP group than in the non-coated group. Newly formed bone was histologically found around the artificial ligament in the SrSiP group. Conclusions: Our findings demonstrate that artificial ligaments using SrSiP display high osteogenic potential and thus may be efficiently used in future clinical applications.; 博士（医学）・甲第724号・令和元年12月5日; © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.; identifier:BMC musculoskeletal disorders Vol.20 No.1 Article No.396 (2019 Aug); identifier:14712474; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3695; identifier:BMC musculoskeletal disorders, 20(1): Article No.396
• 一次資料へのリンクURL: fulltext; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3695/1/01%e7%94%b2724%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3695/2/02%e7%94%b2724%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf; http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3695/3/03%e7%94%b2724%e6%9c%ac%e6%96%87.pdf
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1186/s12891-019-2777-8; https://doi.org/10.1186/s12891-019-2777-8
• 掲載誌（NCID）: AA12035278; https://ci.nii.ac.jp/ncid/AA12035278
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU