Morphine inhibits cell viability and growth via suppression of vascular endothelial growth factor in human oral cancer HSC-3 cells.

• 資料種別: 博士論文
• タイトル: Morphine inhibits cell viability and growth via suppression of vascular endothelial growth factor in human oral cancer HSC-3 cells.
• 著者・編者: Nishiwada, Tadashi; Kawaraguchi, Yoshitaka; Uemura, Keiko; Kawaguchi, Masahiko
• 著者標目: Nishiwada, Tadashi; Kawaraguchi, Yoshitaka; Uemura, Keiko; Kawaguchi, Masahiko
• 出版事項: Springer Nature
• 出版年月日等: 2019-06
• 出版年（W3CDTF）: 2019-06
• 並列タイトル等: モルヒネは血管内皮増殖因子VEGFを抑制することによって、口腔癌細胞HSC-3細胞の活性および細胞増殖を阻害する
• タイトル（掲載誌）: Journal of anesthesia
• 巻号年月日等（掲載誌）: 33 3
• 掲載巻: 33
• 掲載号: 3
• 掲載ページ: 408-415
• ISSN（掲載誌）: ISSN : 0913-8668
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2020-03-16
• 報告番号: 甲第727号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Morphine; HSC-3; Proliferation; Nuclear factor kappa B; Vascular endothelial growth factor
• 対象利用者: 一般
• 一般注記: type:Thesis; Purpose: Although many oral cancer patients require opioids, the effects of morphine and related drugs on oral cancer progression have not been well established. Thus, we examined the effects of morphine exposure on the viability of human oral squamous carcinoma HSC-3 cells and aimed to identify the underlying mechanism. Methods: We exposed HSC-3 cells to the various concentrations of morphine (0, 0.1, 1, 10, 100, or 1000 μmol/L) for 48 h and, subsequently, evaluated cell viability using the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay and cytotoxicity using the lactate dehydrogenase (LDH) assay. To explore the effects of morphine on cell proliferation further, colony formation assay and cell cycle analysis were performed. Additionally, the intracellular expression of nuclear factor kappa B (NF-κB) was analyzed using flow cytometry, and vascular endothelial growth factor (VEGF)-A was evaluated using human VEGF assay. Results: Morphine exposure reduced cell viability and enhanced cytotoxicity in HSC-3 cells in a concentration-dependent manner. The number of colonies in the morphine-treated groups was significantly lower than that in the control group. Consistent with these results, morphine exposure significantly reduced the concentration of VEGF in the cell culture medium in a concentration-dependent manner. However, our data show that morphine at clinical concentrations (0.1-10 μmol/L) does not affect cell cycle and apoptosis. Conclusions: Our results suggest that in human oral cancer HSC-3 cells, morphine exposure inhibits cell viability and growth via suppression of VEGF in clinical conditions.; 博士（医学）・甲第727号・令和2年3月16日; © Japanese Society of Anesthesiologists 2019; This is a post-peer-review, pre-copyedit version of an article published in Journal of anesthesia. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00540-019-02645-1.; identifier:Journal of anesthesia Vol.33 No.3 p.408-415 (2019 Jun); identifier:09138668; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3718; identifier:Journal of anesthesia, 33(3): 408-415
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1007/s00540-019-02645-1
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3718/1/01%e7%94%b2727%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3718/2/02%e7%94%b2727%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3718/3/03%e7%94%b2727%e6%9c%ac%e6%96%87.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3718/4/04%e7%94%b2727Fig1-5.pdf fulltext