Overexpression of Cullin4A correlates with a poor prognosis and tumor progression in esophageal squamous cell carcinoma.

• 資料種別: 博士論文
• タイトル: Overexpression of Cullin4A correlates with a poor prognosis and tumor progression in esophageal squamous cell carcinoma.
• 著者・編者: Nakade, Hiroshi; Migita, Kazuhiro; Matsumoto, Sohei; Wakatsuki, Kohei; Kunishige, Tomohiro; Miyao, Shintaro; Sho, Masayuki
• 著者標目: Nakade, Hiroshi; Migita, Kazuhiro; Matsumoto, Sohei; Wakatsuki, Kohei; Kunishige, Tomohiro; Miyao, Shintaro; Sho, Masayuki
• 出版事項: Springer Nature
• 出版年月日等: 2020-03
• 出版年（W3CDTF）: 2020-03
• 並列タイトル等: 食道扁平上皮癌における予後因子および腫瘍進展メカニズムとCullin4A高発現との関連性について
• タイトル（掲載誌）: International journal of clinical oncology
• 巻号年月日等（掲載誌）: 25 3
• 掲載巻: 25
• 掲載号: 3
• 掲載ページ: 446-455
• ISSN（掲載誌）: ISSN : 1341-9625
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2020-03-16
• 報告番号: 乙第1449号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: Cullin4A; Esophageal squamous cell carcinoma; E3 ubiquitin ligase; p21 protein; Recurrence
• 対象利用者: 一般
• 一般注記: type:Thesis; Background: Cullin4A (CUL4A), which is a component of E3 ubiquitin ligase, is implicated in many cellular events. Although the altered expression of CUL4A has been reported in several human cancers, the role of CUL4A in esophageal cancer remains unknown. Methods: We investigated the CUL4A expression in primary esophageal squamous cell carcinoma (ESCC) tissue specimens from 120 patients by immunohistochemistry and explored its clinical relevance and prognostic value. Furthermore, the effect of the expression of CUL4A on cancer cell proliferation was analyzed in vitro using an siRNA silencing technique. Results: The higher expression of CUL4A was significantly associated with a deeper depth of tumor invasion (P < 0.001) and the presence of venous invasion (P = 0.014). The disease-specific survival (DSS) rate in patients with tumors that showed high CUL4A expression levels was significantly lower than that in patients whose tumors showed low CUL4A expression levels (P = 0.001). Importantly, the CUL4A status was identified as an independent prognostic factor for DSS (P = 0.045). Our results suggested that the CUL4A expression has significant prognostic value in ESCC. Furthermore, CUL4A gene silencing significantly inhibited the proliferation of ESCC cells in vitro. In addition, the knockdown of the CUL4A expression induced G1 phase arrest and increased the p21 and p27 protein levels. Conclusions: CUL4A might play an important role in regulating the proliferation of ESCC cells and promoting the development of postoperative recurrence.; 博士（医学）・乙第1449号・令和2年3月16日; © Japan Society of Clinical Oncology 2019; This is a post-peer-review, pre-copyedit version of an article published in International journal of clinical oncology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10147-019-01547-2.; identifier:International journal of clinical oncology Vol.25 No.3 p.446-455 (2020 Mar); identifier:13419625; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3739; identifier:International journal of clinical oncology, 25(3): 446-455
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.1007/s10147-019-01547-2
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

目次

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3739/1/01%e4%b9%991449%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3739/2/02%e4%b9%991449%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3739/6/03%e4%b9%991449%e6%9c%ac%e6%96%87.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3739/7/04%e4%b9%991449Fig1-4.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3739/8/05%e4%b9%991449Table.pdf fulltext