Inhibition of Heparanase Expression Results in Suppression of Invasion, Migration and Adhesion Abilities of Bladder Cancer Cells.

• 資料種別: 博士論文
• タイトル: Inhibition of Heparanase Expression Results in Suppression of Invasion, Migration and Adhesion Abilities of Bladder Cancer Cells.
• 著者・編者: Tatsumi, Yoshihiro; Miyake, Makito; Shimada, Keiji; Fujii, Tomomi; Hori, Shunta; Morizawa, Yosuke; Nakai, Yasushi; Anai, Satoshi; Tanaka, Nobumichi; Konishi, Noboru; Fujimoto, Kiyohide
• 著者標目: Tatsumi, Yoshihiro; Miyake, Makito; Shimada, Keiji; Fujii, Tomomi; Hori, Shunta; Morizawa, Yosuke; Nakai, Yasushi; Anai, Satoshi; Tanaka, Nobumichi; Konishi, Noboru; Fujimoto, Kiyohide
• 出版事項: MDPI
• 出版年月日等: 2020-05-27
• 出版年（W3CDTF）: 2020-05-27
• 並列タイトル等: 膀胱癌細胞株において、ヘパラナーゼを阻害することにより、細胞浸潤、遊走、接着能を抑制する
• タイトル（掲載誌）: International journal of molecular sciences
• 巻号年月日等（掲載誌）: 21 11
• 掲載巻: 21
• 掲載号: 11
• 掲載ページ: Article No.3789-
• ISSN（掲載誌）: ISSN : 1422-0067
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2021-03-15
• 報告番号: 乙第1506号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: heparanase; syndecan-1; heparan sulfate proteoglycans (HSPGs); urothelial carcinoma
• 対象利用者: 一般
• 一般注記: type:Thesis; Heparan sulfate proteoglycan syndecan-1, CD138, is known to be associated with cell proliferation, adhesion, and migration in malignancies. We previously reported that syndecan-1 (CD138) may contribute to urothelial carcinoma cell survival and progression. We investigated the role of heparanase, an enzyme activated by syndecan-1 in human urothelial carcinoma. Using human urothelial cancer cell lines, MGH-U3 and T24, heparanase expression was reduced with siRNA and RK-682, a heparanase inhibitor, to examine changes in cell proliferation activity, induction of apoptosis, invasion ability of cells, and its relationship to autophagy. A bladder cancer development mouse model was treated with RK-682 and the bladder tissues were examined using immunohistochemical analysis for Ki-67, E-cadherin, LC3, and CD31 expressions. Heparanase inhibition suppressed cellular growth by approximately 40% and induced apoptosis. The heparanase inhibitor decreased cell activity in a concentration-dependent manner and suppressed invasion ability by 40%. Inhibition of heparanase was found to suppress autophagy. In N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN)-induced bladder cancer mice, treatment with heparanase inhibitor suppressed the progression of cancer by 40%, compared to controls. Immunohistochemistry analysis showed that heparanase inhibitor suppressed cell growth, and autophagy. In conclusion, heparanase suppresses apoptosis and promotes invasion and autophagy in urothelial cancer.; 博士（医学）・乙第1506号・令和3年3月15日; © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).; identifier:International journal of molecular sciences Vol.21 No.11 Article No.3789 (2020 May); identifier:14220067; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3932; identifier:International journal of molecular sciences, 21(11): Article No.3789
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（DOI）: 10.3390/ijms21113789
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 奈良県立医科大学 : 奈良県立医科大学機関リポジトリ GINMU

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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3932/1/01%e4%b9%991506%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3932/2/02%e4%b9%991506%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf fulltext

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3932/3/03%e4%b9%991506%e6%9c%ac%e6%96%87.pdf fulltext