Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein

• 資料種別: 博士論文
• タイトル: Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein
• 著者・編者: Rajabali, Zadeh Vahid
• 著者標目: Rajabali, Zadeh Vahid
• 出版事項: Microbiology Society
• 出版年月日等: 2020-09-18
• 出版年（W3CDTF）: 2020-09-18
• 並列タイトル等: ヒトBST-2/tetherinはフニンウイルスの宿主細胞からの放出を阻害し、その阻害作用はウイルス核タンパク質により部分的に相殺される。
• タイトル（掲載誌）: Journal of General Virology
• 巻号年月日等（掲載誌）: 101 6
• 掲載巻: 101
• 掲載号: 6
• 掲載ページ: 573-586
• ISSN（掲載誌）: ISSN : 0022-1317; ISSN : 1465-2099
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2020-09-18
• 報告番号: 甲医歯薬第1266号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: Junin virus; Arenaviridae; human BST-2; innate immunity; Tetherin
• 対象利用者: 一般
• 一般注記: Bone marrow stromal cell antigen-2 (BST-2), also known as tetherin, is an interferon-inducible membrane-associated protein. It effectively targets enveloped viruses at the release step of progeny viruses from host cells, thereby restricting the further spread of viral infection. Junin virus (JUNV) is a member of Arenaviridae, which causes Argentine haemorrhagic fever that is associated with a high rate of mortality. In this study, we examined the effect of human BST-2 on the replication and propagation of JUNV. The production of JUNV Z-mediated virus-like particles (VLPs) was significantly inhibited by over-expression of BST-2. Electron microscopy analysis revealed that BST-2 functions by forming a physical link that directly retains VLPs on the cell surface. Infection using JUNV showed that infectious JUNV production was moderately inhibited by endogenous or exogenous BST-2. We also observed that JUNV infection triggers an intense interferon response, causing an upregulation of BST-2, in infected cells. However, the expression of cell surface BST-2 was reduced upon infection. Furthermore, the expression of JUNV nucleoprotein (NP) partially recovered VLP production from BST-2 restriction, suggesting that the NP functions as an antagonist against antiviral effect of BST-2. We further showed that JUNV NP also rescued the production of Ebola virus VP40-mediated VLP from BST-2 restriction as a broad spectrum BST-2 antagonist. To our knowledge, this is the first report showing that an arenavirus protein counteracts the antiviral function of BST-2.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1266号 学位授与年月日:令和2年9月18日; Author: Vahid Rajabali Zadeh?, Shuzo Urata?, Miako Sakaguchi, Jiro Yasuda?; Citation: Journal of General Virology, 101(6), pp.573-586; 2020; identifier:Nagasaki University (長崎大学), 博士(医学) (2020-09-18)
• 一次資料へのリンクURL: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=47&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=47&item_no=1&attribute_id=70&file_no=1
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: c 2020 The Authors. The definitive peer reviewed, edited version of this article is published in Journal of General Virology, 101(6), 2020, https://doi.org/10.1099/jgv.0.001414.
• 関連情報: http://hdl.handle.net/10069/40270
• 関連情報（DOI）: 10.1099/jgv.0.001414
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 長崎大学 : 長崎大学学術研究成果リポジトリ