並列タイトル等リン酸オクタカルシウムコラーゲン複合体を担体とした低用量rhBMP-2による骨再生
タイトル（掲載誌）Journal of Hard Tissue Biology
授与機関名Nagasaki University (長崎大学)
一般注記Bone morphogenetic protein-2 (BMP-2) has diverse functions and is especially important in bone and cartilage development. Recombinant human BMP-2 (rhBMP-2) is an osteoinductive growth factor that has been clinically applied as a bone graft substitute. However, high-dose rhBMP-2 can cause complications such as induction of significant swelling that can endanger the patient’s life. Atelocollagen sponge (ACS) is the commercially provided standard carrier of rhBMP-2 in clinical applications. However, a large concentration of rhBMP-2 is required to be clinically effective with ACS as the carrier. Octacalcium phosphate/collagen (OCP/Col) has been shown to be an excellent bone substitute compared with other bone substitute materials such as hydroxyapatite or β-tricalcium phosphate due to its biological properties. In this study, we evaluated the use of OCP/Col as a carrier to minimize the effective dose of rhBMP-2. ACS or OCP/Col discs impregnated with different rhBMP-2 concentrations were implanted in mice calvarial bone defects. Morphological analysis with micro-CT both at 4 and 6 weeks post-implantation showed homogenous hard tissue formation in the defects of the OCP/Col group at all rhBMP-2 concentrations tested (0, 0.25, 0.50, or 1.00 μg). In contrast, ACS alone or with 0.25 μg of rhBMP-2 showed almost no bone formation. However, bone mineral density in all groups of ACS and OCP/Col was not dependent on rhBMP-2 concentration. Histological evaluation indicated that bone formation progressed depending on rhBMP-2 concentration in the defects of both the ACS and OCP/Col groups, although the newly formed bone area was significantly higher in the OCP/Col group than in the ACS group. These results indicate that OCP/Col could be an effective carrier of rhBMP-2, minimizing the application dose of rhBMP-2 in clinical settings and avoiding the complications caused by high-dose rhBMP-2.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1297号 学位授与年月日:令和3年3月22日
Author: Nguyen Dien Bien, Kei-ichiro Miura, Yoshinori Sumita, Yuya Nakatani, Rena Shido, Fumihiko Kajii, Shinji Kamakura and Izumi Asahina
Citation: Journal of Hard Tissue Biology, 29(2), pp.123-130; 2020
identifier:Nagasaki University (長崎大学), 博士(歯学) (2021-03-22)
一次資料へのリンクURLhttps://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=26368&item_no=1&attribute_id=70&file_no=1 （fulltext）
著作権情報© 2020 by The Hard Tissue Biology Network Association (JHTBNet)
関連情報http://hdl.handle.net/10069/00040562
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）