並列タイトル等Bio-3Dプリンターで作製したscaffold freeの管状構造体を用いた胆管再建
授与機関名Nagasaki University (長崎大学)
一般注記Introduction: Biliary strictures after bile duct injury or duct-to-duct biliary reconstruction are serious complications that markedly reduce patients’ quality of life because their treatment involves periodic stent replacements. This study aimed to create a scaffold-free tubular construct as an interposition graft to treat biliary complications.Methods: Scaffold-free tubular constructs of allogeneic pig fibroblasts, that is, fibroblast tubes, were created using a Bio-3D Printer and implanted into pigs as interposition grafts for duct-to-duct biliary reconstruction.Results: Although the fibroblast tube was weaker than the native bile duct, it was sufficiently strong to enable suturing. The pigs' serum hepatobiliary enzyme levels remained stable during the experimental period. Micro-computed tomography showed no biliary strictures, no biliary leakages, and no intrahepatic bile duct dilations. The tubular structure was retained in all resected specimens, and the fibroblasts persisted at the graft sites. Immunohistochemical analyses revealed angiogenesis in the fibroblast tube and absence of extensions of the biliary epithelium into the fibroblast tube's lumen.Conclusions: This study's findings demonstrated successful reconstruction of the extrahepatic bile duct with a scaffold-free tubular construct created from pig fibroblasts using a novel Bio-3D Printer. This construct could provide a novel regenerative treatment for patients with hepatobiliary diseases.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1360号 学位授与年月日:令和3年9月1日
Author: Takashi Hamada, Anna Nakamura, Akihiko Soyama, Yusuke Sakai, Takayuki Miyoshi, Shun Yamaguchi, Masaaki Hidaka, Takanobu Hara, Tota Kugiyama, Mitsuhisa Takatsuki, Akihide Kamiya, Koichi Nakayama, Susumu Eguchi
Citation: Regenerative Therapy, 16, pp.81-89; 2021
identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-01)
一次資料へのリンクURLhttps://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27085&item_no=1&attribute_id=70&file_no=1 （fulltext）
著作権情報© 2021, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
関連情報http://hdl.handle.net/10069/00040946
関連情報（DOI）10.1016/j.reth.2021.02.001
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）