Expression of N-terminal deficient E-cadherin protein in invasive lobular carcinoma of the breast

• 資料種別: 博士論文
• タイトル: Expression of N-terminal deficient E-cadherin protein in invasive lobular carcinoma of the breast
• 著者・編者: 安井, 春奈
• 著者標目: 安井, 春奈
• 出版事項: Wolters Kluwer Health, Inc.
• 出版年月日等: 2021-12-01
• 出版年（W3CDTF）: 2021-12-01
• 並列タイトル等: 乳腺浸潤性小葉癌におけるN末端欠損E-カドヘリン蛋白の発現
• タイトル（掲載誌）: The American Journal of Surgical Pathology
• ISSN（掲載誌）: ISSN : 0147-5185; ISSN : 1532-0979
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2021-12-01
• 報告番号: 甲医歯薬第1375号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: breast cancer; Invasive lobular carcinoma; E-cadherin; N-terminal; C-terminal
• 対象利用者: 一般
• 一般注記: Invasive lobular carcinoma (ILC) of the breast is characterized by the discohesive growth of tumor cells, which is mainly associated with the complete loss of E-cadherin (E-cad) expression. However, some aberrant expression patterns of E-cad protein that are inconsistent with their morphologies have been reported in ILC. We report herein ILC cases expressing a new type of abnormal E-cad protein that lacks the N-terminal domain, but conserves the C-terminal domain on the cell membrane. Immunohistochemical staining of 299 ILC cases using specific antibodies against the N-terminal or C-terminal region of E-cad revealed that 227 (76%) cases showed loss of the membranous expression of both terminuses (N−/C−) and 72 (24%) cases showed expression of only the C-terminus (N−/C+). In all cases, the expression of p120-catenin and β-catenin coincided with the expression of the C-terminus of E-cad. Clinicopathologic analysis revealed that N−/C+ expression in ILC cells was significantly associated with the histologic subtype (especially mixed-type ILC with another histologic type) and immunohistochemical molecular subtype (especially the triple-negative subtype), but not with prognostic factors (pT or pN). In addition, 12 of 15 cases (80%) with aberrant cytoplasmic localization of the N-terminal of E-cad showed diffuse membranous expression of the C-terminal domain. Additional immunohistochemistry using an antibody recognizing the extracellular juxtamembrane region showed that 28 (39%) of the N−/C+ cases had lost membranous expression, suggesting diversity in the deletion pattern of the N-terminal region. Our findings provide a novel mechanism for the loss of E-cad function because of N-terminal-deficient E-cad protein in ILC.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1375号 学位授与年月日:令和3年12月1日; Author: Haruna Yasui, Takuya Kawata, Koji Muramatsu, Yuko Kakuda, Takuma Oishi, Tomoko Norose, Akifumi Notsu, Seiichiro Nishimura, Junya Fukuoka, Takashi Sugino; Citation: The American Journal of Surgical Pathology, doi: 10.1097/PAS.0000000000001822; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01)
• 一次資料へのリンクURL: https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27100&item_no=1&attribute_id=70&file_no=1 （fulltext）; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27100&item_no=1&attribute_id=70&file_no=1
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: © 2021 Wolters Kluwer Health, Inc. All rights reserved. This is a non-final version of an article published in final form in The American Journal of Surgical Pathology, doi:10.1097/PAS.0000000000001822; 2021.
• 関連情報: http://hdl.handle.net/10069/00041136
• 関連情報（DOI）: 10.1097/PAS.0000000000001822
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 長崎大学 : 長崎大学学術研究成果リポジトリ